Altafini Alberto, Tassinari Marco, Guerrini Alessandro, Roncada Paola
Department of Veterinary Medical Sciences, University of Bologna, Via Tolara di Sopra 50, 40064 Ozzano dell'Emilia (Bologna), Italy.
Vet Sci. 2020 Nov 12;7(4):176. doi: 10.3390/vetsci7040176.
Aflatoxin M (AFM) is a well-known mycotoxin that can be found in the milk of animals that have ingested feed contaminated with aflatoxin B (AFB). In Italy, the development of donkey farms is mainly due to growing request of donkey milk, which is considered an incomparable substitute for human mother's milk for its chemical composition and organoleptic characteristics. The aim of this study was to assess the occurrence of AFM in donkey milk produced in a farm in Northern Italy, also in view of the few data available about the presence of this mycotoxin in this type of milk. Therefore, 63 milk samples were collected and analyzed using a fast and sensitive HPLC and fluorescence detection (FLD) method previously optimized and validated. None of the milk samples collected were found to be contaminated at a level above the limit of quantification (LOQ) (0.0125 ng/mL), while only one sample showed traces of the mycotoxin at a concentration between the limit of detection (LOD) and LOQ (0.0044 ng/mL), well below the legal limit established for infant milk and follow-on milk (0.025 ng/mL). These results are in line with those of the few similar surveys carried out on donkey milk and seem to indicate a low risk of AFM contamination for this food.
黄曲霉毒素M（AFM）是一种著名的霉菌毒素，可在摄入被黄曲霉毒素B（AFB）污染饲料的动物的乳汁中发现。在意大利，驴奶场的发展主要归因于对驴奶需求的不断增长，驴奶因其化学成分和感官特性被认为是人类母乳无与伦比的替代品。本研究的目的是评估意大利北部一个农场生产的驴奶中AFM的存在情况，鉴于关于这种霉菌毒素在这类牛奶中存在情况的可用数据较少。因此，收集了63份牛奶样本，并使用先前优化和验证的快速灵敏的高效液相色谱法和荧光检测（FLD）方法进行分析。所收集的牛奶样本中没有一份被发现污染水平高于定量限（LOQ）（0.0125纳克/毫升），而只有一个样本显示出痕量的霉菌毒素，浓度在检测限（LOD）和LOQ之间（0.0044纳克/毫升），远低于为婴儿奶粉和后续奶粉设定的法定限量（0.025纳克/毫升）。这些结果与对驴奶进行的少数类似调查结果一致，似乎表明这种食品受AFM污染的风险较低。
