College of Agronomy, Northwest A&F University, Yangling, 712100, Shaanxi, China.
School of Biological and Chemical Engineering, Panzhihua University, Panzhihua, 617000, Sichuan, China.
Sci Rep. 2020 Nov 16;10(1):19911. doi: 10.1038/s41598-020-77044-8.
Fungal communities are considered to be critically important for crop health and soil fertility. However, our knowledge of the response of fungal community structure to the continuous cropping of flue-cured tobacco is limited, and the interaction of soil fungal communities under different cropping systems remains unclear. In this study, we comparatively investigated the fungal abundance, diversity, and community composition in the soils in which continuous cropping of flue-cured tobacco for 3 years (3ys), 5 years (5ys), and cropping for 1 year (CK) using quantitative polymerase chain reaction and high-throughput sequencing technology. The results revealed that continuous cropping of flue-cured tobacco changed the abundance of soil fungi, and caused a significant variation in fungal diversity. In particular, continuous cropping increased the relative abundance of Mortierellales, which can dissolve mineral phosphorus in soil. Unfortunately, continuous cropping also increased the risk of potential pathogens. Moreover, long-term continuous cropping had more complex and stabilize network. This study also indicated that available potassium and available phosphorous were the primary soil factors shifting the fungal community structure. These results suggested that several soil variables may affect fungal community structure. The continuous cropping of flue-cured tobacco significantly increased the abundance and diversity of soil fungal communities.
真菌群落被认为对作物健康和土壤肥力至关重要。然而，我们对真菌群落结构对烤烟连作的响应的了解有限，不同种植制度下土壤真菌群落的相互作用仍不清楚。本研究采用定量聚合酶链反应和高通量测序技术，比较研究了连续种植烤烟 3 年（3ys）、5 年（5ys）和种植 1 年（CK）的土壤中真菌的丰度、多样性和群落组成。结果表明，烤烟连作对土壤真菌的丰度有影响，并且对真菌多样性有显著的变化。特别是，连续种植增加了可以溶解土壤中矿物磷的 Mortierellales 的相对丰度。不幸的是，连续种植也增加了潜在病原体的风险。此外，长期连作具有更复杂和稳定的网络。本研究还表明，有效钾和有效磷是改变真菌群落结构的主要土壤因素。这些结果表明，一些土壤变量可能会影响真菌群落结构。烤烟连作显著增加了土壤真菌群落的丰度和多样性。
