Mahmoudvand Hossein, Al-Abodi Hiba Riyadh, Zolfagharkhani Peyman, Ghasemian Yadegari Javad
Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran.
Department of Environment, College of Science, University of Al-Qadisiyah, Al-Diwaniyah, Iraq.
J Parasit Dis. 2022 Dec;46(4):1047-1054. doi: 10.1007/s12639-022-01517-y. Epub 2022 Aug 28.
We aimed to investigate the and anti-helminthic effects of chloroform extract (AECE) as well as its cellular mechanisms against protoscoleces. and anti-helminthic effects of AECE on protoscoleces were evaluated through eosin exclusion test. Effects of AECE on induction of the caspase-3 like activity, the permeability of plasma membrane were also determined. The effects of AECE against the human hepatocyte carcinoma (HepG2) and human embryonic kidney (HEK293T) cells were determined by MTT ([3-(4.5-dimethylthiazol-2-yl)-2.5-diphenyl tetrazolium bromide)]) test. The findings revealed that AECE especially at 45 mg/mL displayed potent and anti-helminthic effects against protoscoleces ( < 0.001). After treatment of protoscoleces with AECE, the caspase-3 enzyme activity and the rate of permeability of plasma membrane was dose dependently ( < 0.001) increased. The 50% cytotoxic concentration (CC) value of AECE for normal and cancer cells lines 4.62 mg/mL and 1.89 mg/mL, respectively. The findings of the current study revealed the favorable and anti-helminthic effects of AECE against protoscoleces of . However, further surveys in animal model are required to elucidate different aspects of this extract before use in clinical phases.
我们旨在研究氯仿提取物（AECE）的驱虫和抗蠕虫作用及其针对原头节的细胞机制。通过伊红排斥试验评估AECE对原头节的驱虫和抗蠕虫作用。还测定了AECE对caspase-3样活性诱导、质膜通透性的影响。通过MTT（[3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐]）试验测定AECE对人肝癌细胞（HepG2）和人胚肾细胞（HEK293T）的作用。研究结果显示，AECE尤其是在45mg/mL时对原头节表现出强大的驱虫和抗蠕虫作用（P<0.001）。用AECE处理原头节后，caspase-3酶活性和质膜通透性速率呈剂量依赖性增加（P<0.001）。AECE对正常细胞系和癌细胞系的50%细胞毒性浓度（CC）值分别为4.62mg/mL和1.89mg/mL。本研究结果揭示了AECE对原头节具有良好的驱虫和抗蠕虫作用。然而，在临床阶段使用之前，需要在动物模型中进行进一步研究以阐明该提取物的不同方面。
