Anti-helminthic effects and cellular mechanisms of extract against protoscoleces.

We aimed to investigate the and anti-helminthic effects of chloroform extract (AECE) as well as its cellular mechanisms against protoscoleces. and anti-helminthic effects of AECE on protoscoleces were evaluated through eosin exclusion test. Effects of AECE on induction of the caspase-3 like activity, the permeability of plasma membrane were also determined. The effects of AECE against the human hepatocyte carcinoma (HepG2) and human embryonic kidney (HEK293T) cells were determined by MTT ([3-(4.5-dimethylthiazol-2-yl)-2.5-diphenyl tetrazolium bromide)]) test. The findings revealed that AECE especially at 45 mg/mL displayed potent and anti-helminthic effects against protoscoleces ( < 0.001). After treatment of protoscoleces with AECE, the caspase-3 enzyme activity and the rate of permeability of plasma membrane was dose dependently ( < 0.001) increased. The 50% cytotoxic concentration (CC) value of AECE for normal and cancer cells lines 4.62 mg/mL and 1.89 mg/mL, respectively. The findings of the current study revealed the favorable and anti-helminthic effects of AECE against protoscoleces of . However, further surveys in animal model are required to elucidate different aspects of this extract before use in clinical phases.