IEEE Trans Biomed Eng. 2021 Aug;68(8):2368-2376. doi: 10.1109/TBME.2020.3038466. Epub 2021 Jul 16.
3D optical coherence tomography (OCT) is used for analyses of human placenta organoids in situ without sample preparation.
The trophoblast organoids analyzed were derived from primary human trophoblast. In this study a custom made ultra-high-resolution spectral domain OCT system with uniform spatial and axial resolution of 2.48 μm in organoid tissue was used. The obtained OCT results align to differentiation status tested via quantitative polymerase chain reaction, Western blot analyses, immunohistochemistry, and immunofluorescence of histological sections.
3D OCT enables a more detailed placenta organoid monitoring compared to brightfield microscopy. Inner architecture with light scattering "bridges" surrounding cavities were visualized and quantified in situ for the first time. The formation of these bridges and cavities is congruent to differentiated trophoblast organoids having developed syncytiotrophoblasts.
Using 3D OCT in living placenta organoids is a fast tool to assess the differentiation status and resolve internal structures in situ, which is not possible with standard live cell imaging modality.
Only recently human placenta-derived organoids were established, allowing to have a highly reproducible and stable in vitro model to investigate not only developmental but also physiological and pathophysiological processes during early pregnancy. To our knowledge, this work is the first to analyze living human placenta organoids using 3D OCT. Thereby, the rapid and especially non-endpoint OCT qualitative analyses align to the differentiation stage of organoids, which will aid future advancement in this field.
3D 光学相干断层扫描（OCT）可用于在无需样品制备的情况下原位分析人胎盘类器官。
分析的滋养层类器官源自原代人滋养层。在这项研究中，使用了具有均匀空间和轴向分辨率为 2.48μm 的定制超高分辨率光谱域 OCT 系统。所获得的 OCT 结果与通过定量聚合酶链反应、Western blot 分析、免疫组织化学和组织学切片的免疫荧光测试的分化状态相吻合。
与明场显微镜相比，3D OCT 能够更详细地监测胎盘类器官。首次可视化和原位量化了具有散射“桥”的内部结构，这些桥围绕着腔室。这些桥和腔室的形成与分化的滋养层类器官形成合胞滋养层相一致。
在活胎盘类器官中使用 3D OCT 是一种快速评估分化状态并原位解析内部结构的工具，这是标准活细胞成像方式无法实现的。
仅在最近才建立了人胎盘来源的类器官，这使得能够建立一个高度可重复且稳定的体外模型，不仅可以研究发育过程，还可以研究早期妊娠期间的生理和病理生理过程。据我们所知，这项工作首次使用 3D OCT 分析活的人胎盘类器官。因此，快速且特别是非终点 OCT 定性分析与类器官的分化阶段相吻合，这将有助于该领域的未来发展。
