Institute of Ageing and Chronic Disease , University of Liverpool , Liverpool L7 8TX , U.K.
Institute of Integrative Biology , University of Liverpool , Liverpool L69 7ZB , U.K.
J Proteome Res. 2018 Nov 2;17(11):3780-3790. doi: 10.1021/acs.jproteome.8b00455. Epub 2018 Oct 3.
Despite osteoarthritis (OA) and rheumatoid arthritis (RA) being typically age-related, their underlying etiologies are markedly different. We used H nuclear magnetic resonance (NMR) spectroscopy to identify differences in metabolite profiles in low volumes of OA and RA synovial fluid (SF). SF was aspirated from knee joints of 10 OA and 14 RA patients. 100 μL SF was analyzed using a 700 MHz Avance IIIHD Bruker NMR spectrometer with a TCI cryoprobe. Spectra were analyzed by Chenomx, Bruker TopSpin and AMIX software. Statistical analysis was undertaken using Metaboanalyst. 50 metabolites were annotated, including amino acids, saccharides, nucleotides and soluble lipids. Discriminant analysis identified group separation between OA and RA cohorts, with 32 metabolites significantly different between OA and RA SF (false discovery rate (FDR) < 0.05). Metabolites of glycolysis and the tricarboxylic acid cycle were lower in RA compared to OA; these results concur with higher levels of inflammation, synovial proliferation and hypoxia found in RA compared to OA. Elevated taurine in OA may indicate increased subchondral bone sclerosis. We demonstrate that quantifiable differences in metabolite abundance can be measured in low volumes of SF by H NMR spectroscopy, which may be clinically useful to aid diagnosis and improve understanding of disease pathogenesis.
尽管骨关节炎（OA）和类风湿关节炎（RA）通常与年龄有关，但它们的潜在病因明显不同。我们使用 H 核磁共振（NMR）光谱技术来识别少量 OA 和 RA 滑膜液（SF）中代谢物谱的差异。从 10 名 OA 和 14 名 RA 患者的膝关节中抽吸 SF。使用配备 TCI 冷探头的 700 MHz Avance IIIHD Bruker NMR 光谱仪分析 100 μL SF。使用 Chenomx、Bruker TopSpin 和 AMIX 软件对光谱进行分析。使用 Metaboanalyst 进行统计分析。注释了 50 种代谢物，包括氨基酸、糖、核苷酸和可溶性脂质。判别分析确定了 OA 和 RA 队列之间的分组分离，OA 和 RA SF 之间有 32 种代谢物存在显著差异（错误发现率（FDR）<0.05）。与 OA 相比，RA 中的糖酵解和三羧酸循环代谢物较低；这些结果与 RA 中比 OA 更高的炎症水平、滑膜增殖和缺氧相一致。OA 中的牛磺酸升高可能表明骨下骨硬化增加。我们证明，通过 H NMR 光谱可以在少量 SF 中测量可量化的代谢物丰度差异，这可能对辅助诊断和提高对疾病发病机制的理解具有临床意义。
