益生菌补充对人乳中免疫细胞表面标志物基因表达、抗体和促炎细胞因子水平的影响。
The effects of probiotic supplementation on the gene expressions of immune cell surface markers and levels of antibodies and pro-inflammatory cytokines in human milk.
机构信息
Department of Neonatal Immunology and Microbiology, Medolac Laboratories A Public Benefit Corporation, Boulder City, NV, USA.
出版信息
J Perinatol. 2021 May;41(5):1083-1091. doi: 10.1038/s41372-020-00875-w. Epub 2020 Nov 18.
OBJECTIVE
This study investigated the impact of probiotic supplementation on the gene expressions of cluster of differentiation (CD) as cell markers and the concentrations of antibodies and cytokines in human milk.
STUDY DESIGN
Gene expressions of CD28, CD19, and CD38 were determined in milk from 15 women ingesting daily probiotics (from Greek yogurt) and 12 women who do not consume probiotics. Concentrations of antibodies and cytokines were measured using ELISA.
RESULTS
Gene expression of CD28 tended to be higher in milk from mothers ingesting daily probiotics than mothers who did not take probiotics. Interleukin-6 (IL-6) concentration in milk was higher in mothers ingesting probiotics than those who do not consume probiotics. The increase of IL-6 level in human milk was positively correlated with total IgA and IgG concentrations.
CONCLUSIONS
Probiotic supplementation could enhance the secretion of IL-6 in human milk. Human milk IL-6 may improve neonatal immunity due to its stimulation of total IgA and IgG.
目的
本研究旨在探讨益生菌补充对分化群（CD）作为细胞标志物的基因表达以及人乳中抗体和细胞因子浓度的影响。
研究设计
通过测定 15 名每日摄入益生菌（希腊酸奶）的女性和 12 名不摄入益生菌的女性乳汁中的 CD28、CD19 和 CD38 的基因表达，来确定其是否有差异。采用 ELISA 法检测抗体和细胞因子的浓度。
结果
与不摄入益生菌的母亲相比，每日摄入益生菌的母亲乳汁中 CD28 的基因表达呈上升趋势。摄入益生菌的母亲乳汁中白细胞介素 6（IL-6）的浓度高于不摄入益生菌的母亲。人乳中 IL-6 水平的升高与总 IgA 和 IgG 浓度呈正相关。
结论
益生菌补充可能会增加人乳中 IL-6 的分泌。人乳中的 IL-6 可能通过刺激总 IgA 和 IgG 来改善新生儿的免疫力。
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