酿酒酵母中PHO系统的负调控因子：PHO85的分离与结构表征
Negative regulators of the PHO system in Saccharomyces cerevisiae: isolation and structural characterization of PHO85.
作者信息
Uesono Y, Tanaka K, Toh-e A
机构信息
Department of Fermentation Technology, Hiroshima University, Japan.
出版信息
Nucleic Acids Res. 1987 Dec 23;15(24):10299-309. doi: 10.1093/nar/15.24.10299.
One of the negative regulators of the PHO system of Saccharomyces cerevisiae, PHO85, has been isolated by transformation and complementation of a pho85 strain. The complementing activity was delimited within a 1258 bp DNA segment and this region has been sequenced. The largest open reading frame found in this region can encode a protein of 302 amino acid residues. A pho85 mutant resulted from disruption of the chromosomal counterpart of the open reading frame described above. Therefore, we concluded that the gene we have cloned is PHO85. This result also indicates that PHO85 is nonessential. Northern analysis revealed that the size of the PHO85 message is 1.1 kb. No similarity was found between the putative amino acid sequences of two negative regulators, the PHO80 and PHO85 proteins.
通过对pho85菌株进行转化和互补，分离出了酿酒酵母PHO系统的负调控因子之一PHO85。互补活性定位于一段1258 bp的DNA片段内，并且对该区域进行了测序。在该区域发现的最大开放阅读框可编码一个由302个氨基酸残基组成的蛋白质。上述开放阅读框的染色体对应物被破坏后产生了一个pho85突变体。因此，我们得出结论，我们克隆的基因是PHO85。该结果还表明PHO85并非必需基因。Northern分析显示，PHO85信使核糖核酸的大小为1.1 kb。在两个负调控因子PHO80和PHO85蛋白的推定氨基酸序列之间未发现相似性。
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