构巢曲霉次生代谢产物的化学鉴定与分析
The chemical identification and analysis of Aspergillus nidulans secondary metabolites.
作者信息
Sanchez James F, Wang Clay C C
机构信息
Department of Pharmacology and Pharmaceutical Sciences, School of Pharmacy, University of Southern California, Los Angeles, CA, USA.
出版信息
Methods Mol Biol. 2012;944:97-109. doi: 10.1007/978-1-62703-122-6_6.
Filamentous fungi have long been recognized to be a rich source of secondary metabolites with potential medicinal applications. The recent genomic sequencing of several Aspergillus species has revealed that many secondary metabolite gene clusters are apparently silent under standard laboratory conditions. Several successful approaches have been utilized to upregulate these genes and unearth the corresponding natural products. A straightforward, reliable method to purify and characterize new metabolites therefore should be useful. Details are provided herein on the cultivation of Aspergillus nidulans and the LC/MS analysis of the metabolic profile. Following is an explanation of silica gel chromatography, HPLC, and preparative TLC. Finally, the NMR characterization of previously unknown A. nidulans metabolites is detailed.
丝状真菌长期以来一直被认为是具有潜在药用价值的次生代谢产物的丰富来源。最近对几种曲霉属物种的基因组测序表明，许多次生代谢产物基因簇在标准实验室条件下显然是沉默的。已经采用了几种成功的方法来上调这些基因并挖掘相应的天然产物。因此，一种直接、可靠的纯化和表征新代谢产物的方法应该会很有用。本文提供了关于构巢曲霉培养以及代谢谱的液相色谱/质谱分析的详细信息。以下是硅胶柱色谱、高效液相色谱和制备型薄层色谱的说明。最后，详细介绍了对构巢曲霉以前未知的代谢产物的核磁共振表征。
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