Department of Biotechnology, Quaid-i-Azam University, Islamabad, Pakistan.
Department of Pharmacy, Quaid-i-Azam University, Islamabad, Pakistan.
Chemosphere. 2021 Mar;266:128945. doi: 10.1016/j.chemosphere.2020.128945. Epub 2020 Nov 11.
Widespread applications of nanomaterials in food and health sciences have inevitable toxicological outcomes. Among nanomaterials, copper oxide nanoparticles (CuO NPs) are commonly used in all fields due to its distinctive characteristics. The study was designed to investigate the comparative hepatotoxic effect of green (GNPs) and chemically synthesized (CNPs) CuO NPs on spargue-dawely rats and their offspring. NPs (50 and 100 mg/kg) were orally administered to rats twice a week starting before mating. After birth the parents were continued dosing while pups were only on mother feed. Antioxidant, lipid peroxidation, genotoxicity, and histology were performed on liver tissue. In addition serum biochemistry of parents and offspring was also performed. The levels of catalase, peroxidase, and glutathione were significantly lowered only in CNPs treated parents while lipid peroxidation level was increased in these groups. Maximum genotoxicity (2.3%) in terms of percent tail DNA was observed in parent rats administered with high CNPs dose while other groups did not exhibit significant variation in genetoxic parameters. Gender and dose dependent effects were observed on liver function tests especially ALP and ALT in parents however no obvious differences were observed in offspring. Furthermore, dose dependent dilation and congestion of sinusoids was observed on CNPs administration. In GNPs treated rats and offspring histological alterations were observed. The study concludes that chemically synthesized CuO NPs exhibit dose dependent toxic effects on liver as compared to green synthesized CuO NPs. Furthermore lactation does not play significant role in the hepatotoxicity of offspring though minor oxidative stress was observed only on CNPs administration. The study also shows that pharmacological application of green synthesized NPs can be accomplished due to their biocompatible nature.
纳米材料在食品和健康科学中的广泛应用不可避免地会产生毒理学后果。在纳米材料中，氧化铜纳米颗粒（CuO NPs）由于其独特的特性而被广泛应用于各个领域。本研究旨在研究绿色（GNPs）和化学合成（CNPs）CuO NPs 对斯帕鲁迪尔大鼠及其后代的比较肝毒性作用。NPs（50 和 100mg/kg）每周两次经口给予大鼠，在交配前开始给药。出生后，父母继续给药，而幼崽只吃母奶。对肝脏组织进行抗氧化、脂质过氧化、遗传毒性和组织学检查。此外，还对父母和后代的血清生化指标进行了检测。只有在 CNPs 处理的父母中，过氧化氢酶、过氧化物酶和谷胱甘肽的水平显著降低，而这些组中的脂质过氧化水平增加。在给予高剂量 CNPs 的父母大鼠中，以尾巴 DNA 百分比表示的最大遗传毒性（2.3%），而其他组在遗传毒性参数方面没有明显变化。在父母的肝功能试验中观察到性别和剂量依赖性的影响，尤其是碱性磷酸酶和丙氨酸氨基转移酶，但在后代中没有观察到明显的差异。此外，在给予 CNPs 后观察到窦状隙的剂量依赖性扩张和充血。在 GNPs 处理的大鼠和后代中观察到组织学改变。研究结论表明，与绿色合成的 CuO NPs 相比，化学合成的 CuO NPs 对肝脏具有剂量依赖性的毒性作用。此外，哺乳期对后代的肝毒性没有显著作用，尽管仅在给予 CNPs 时观察到轻微的氧化应激。该研究还表明，由于绿色合成 NPs 的生物相容性，可完成其药理学应用。
