Duke School of Medicine, Department of Neurobiology, Durham, NC 27710, United States.
Duke School of Medicine, Department of Neurobiology, Durham, NC 27710, United States.
Curr Opin Neurobiol. 2020 Dec;65:88-99. doi: 10.1016/j.conb.2020.09.011. Epub 2020 Nov 19.
Detailed quantification of neural dynamics across the entire brain will be the key to genuinely understanding perception and behavior. With the recent developments in microscopy and biosensor engineering, the zebrafish has made a grand entrance in neuroscience as its small size and optical transparency enable imaging access to its entire brain at cellular and even subcellular resolution. However, until recently many neurobiological insights were largely correlational or provided little mechanistic insight into the brain-wide population dynamics generated by diverse types of neurons. Now with increasingly sophisticated behavioral, imaging, and causal intervention paradigms, zebrafish are revealing how entire vertebrate brains function. Here we review recent research that fulfills promises made by the early wave of technical advances. These studies reveal new features of brain-wide neural processing and the importance of integrative investigation and computational modelling. Moreover, we outline the future tools necessary for solving broader brain-scale circuit problems.
详细量化整个大脑的神经动力学将是真正理解感知和行为的关键。随着显微镜和生物传感器工程的最新发展，斑马鱼作为一种小型透明动物，已经在神经科学领域崭露头角，因为它可以对整个大脑进行成像，达到细胞甚至亚细胞分辨率。然而，直到最近，许多神经生物学的见解在很大程度上仍然是相关的，或者对不同类型神经元产生的全脑群体动力学提供的机制见解很少。现在，随着越来越复杂的行为、成像和因果干预范式，斑马鱼正在揭示整个脊椎动物大脑的工作原理。在这里，我们回顾了最近的研究，这些研究实现了早期技术进步所带来的承诺。这些研究揭示了全脑神经处理的新特征，以及综合研究和计算建模的重要性。此外，我们还概述了未来解决更广泛的大脑规模电路问题所需的工具。
