Department of Clinical Studies, New Bolton Center, University of Pennsylvania School of Veterinary Medicine, Kennett Square, PA, USA.
Department of Pathobiology, New Bolton Center, University of Pennsylvania School of Veterinary Medicine, Kennett Square, PA, USA.
Equine Vet J. 2021 Nov;53(6):1119-1131. doi: 10.1111/evj.13389. Epub 2020 Dec 14.
Previous studies have identified alterations in the faecal microbiota of horses with colic; however, further work is needed to interpret these findings.
To compare the faecal microbiota of horses presenting for colic at hospital admission, day 1 and day 3/discharge and with different colic duration and lesion locations.
Prospective observational clinical study.
Faecal samples were collected from 17 colic cases at hospital admission, on day 1 and on day 3 post-admission or at the time of hospital discharge if prior to 72 hours. Faecal samples were extracted for genomic DNA, PCR-amplified, sequenced and analysed using QIIME. Species richness and Shannon diversity (alpha diversity) were estimated. The extent of the relationship between bacterial communities (beta diversity) was quantified using pairwise UniFrac distances, visualised using principal coordinate analysis (PCoA) and statistically analysed using permutational multivariate analysis of variance (PERMANOVA). The relative abundance of bacterial populations at the different time points and in different types of colic was compared using ANCOM.
There was a decrease in species richness from admission to day 3/hospital discharge (P < .05), and a lower species richness (P = .005) and Shannon diversity (P = .02) in horses with colic ≥60 h compared to <60 h. Based on PCoA and PERMANOVA, there was a significant difference in bacterial community composition for horses with different colic duration (P = .001) and lesion location (P = .006). Several differences in bacterial phyla and genera were observed at different time points and with different types of colic.
Relatively low numbers and a diverse population of horses.
The microbiota change from hospital admission to day 3/discharge in horses with colic and horses with colic ≥60 h and large colon lesions have a distinct bacterial population compared to horses with colic <60 h and small intestinal lesions.
先前的研究已经确定了患有腹痛的马的粪便微生物群发生了改变；然而，需要进一步的工作来解释这些发现。
比较在医院就诊时、第 1 天和第 3 天/出院时出现腹痛的马的粪便微生物群，以及不同腹痛持续时间和病变部位的马的粪便微生物群。
前瞻性观察性临床研究。
在入院时、入院后第 1 天和第 3 天（如果在 72 小时内出院），从 17 例腹痛病例中采集粪便样本。提取粪便样本进行基因组 DNA 提取、PCR 扩增、测序，并使用 QIIME 进行分析。估计物种丰富度和香农多样性（α多样性）。使用成对的 UniFrac 距离量化细菌群落之间的关系程度（β多样性），使用主坐标分析（PCoA）可视化，并使用置换多元方差分析（PERMANOVA）进行统计分析。使用 ANCOM 比较不同时间点和不同类型腹痛的细菌种群的相对丰度。
从入院到第 3 天/出院时，物种丰富度降低（P<.05），腹痛时间≥60 小时的马的物种丰富度（P=.005）和香农多样性（P=.02）较低。基于 PCoA 和 PERMANOVA，不同腹痛持续时间（P=.001）和病变部位（P=.006）的马的细菌群落组成有显著差异。在不同时间点和不同类型的腹痛中观察到几个细菌门和属的差异。
马匹数量相对较少，种群多样化。
与腹痛持续时间<60 小时和小肠病变的马相比，患有腹痛≥60 小时和大肠病变的马的粪便微生物群从入院到第 3 天/出院时发生了变化。
