Sateriale Daniela, Imperatore Roberta, Colicchio Roberta, Pagliuca Chiara, Varricchio Ettore, Volpe Maria Grazia, Salvatore Paola, Paolucci Marina, Pagliarulo Caterina
Department of Science and Technology, University of Sannio, Benevento, Italy.
Department of Molecular Medicine and Medical Biotechnology, Federico II University, Naples, Italy.
Front Microbiol. 2020 Nov 5;11:592265. doi: 10.3389/fmicb.2020.592265. eCollection 2020.
In the last decades, resistant microbial infection rate has dramatically increased, especially infections due to biofilm-producing strains that require increasingly complex treatments and are responsible for the increased mortality percentages compared with other infectious diseases. Considering that biofilms represent a key factor for a wide range of chronic infections with high drug tolerance, the treatment of biofilm-causing bacterial infections represents a great challenge for the future. Among new alternative strategies to conventional antimicrobial agents, the scientific interest has shifted to the study of biologically active compounds from plant-related extracts with known antimicrobial properties, in order to also evaluate their antibiofilm activity. In this regard, the aim of this study has been to assess the antibiofilm activity of polyphenolic extracts from myrtle leaf and pomegranate peel against oral pathogens of dental plaque, an excellent polymicrobial biofilm model. In particular, the antibiofilm properties of myrtle and pomegranate extracts, also in binary combination, were highlighted. In addition to inhibiting the biofilm formation, the tested polyphenolic extracts have been proven to destroy both preformed single-species and multispecies biofilms formed by , , , and oral isolates, suggesting that the new natural sources are rich in promising compounds able to counteract biofilm-related infections.
在过去几十年中，耐药微生物感染率急剧上升，尤其是由产生生物膜的菌株引起的感染，这些感染需要越来越复杂的治疗方法，并且与其他传染病相比，导致死亡率上升。鉴于生物膜是多种具有高耐药性的慢性感染的关键因素，治疗由生物膜引起的细菌感染对未来构成了巨大挑战。在传统抗菌药物的新替代策略中，科学兴趣已转向研究具有已知抗菌特性的植物相关提取物中的生物活性化合物，以便评估它们的抗生物膜活性。在这方面，本研究的目的是评估桃金娘叶和石榴皮中的多酚提取物对牙菌斑口腔病原体的抗生物膜活性，牙菌斑是一种出色的多微生物生物膜模型。特别是，突出了桃金娘和石榴提取物的抗生物膜特性，包括二元组合时的特性。除了抑制生物膜形成外，测试的多酚提取物已被证明能破坏由、、和口腔分离株形成的预先形成的单菌种和多菌种生物膜，这表明这些新的天然来源富含能够对抗生物膜相关感染 的有前景的化合物。
