Baptista Anderson Barbosa, Sarandy Mariáurea M, Gonçalves Reggiani Vilela, Novaes Rômulo Dias, Gonçalves da Costa Cláudio, Leite João Paulo Viana, Peluzio Maria do Carmo Gouveia
Department of Nutrition and Health, Universidade Federal de Viçosa, Viçosa, Minas Gerais 36570-900, Brazil.
Department of General Biology, Universidade Federal de Viçosa, Viçosa, Minas Gerais 36570-900, Brazil.
Evid Based Complement Alternat Med. 2020 Dec 7;2020:3054521. doi: 10.1155/2020/3054521. eCollection 2020.
The L. (cashew) and D. (cajuí) are plants commonly found in Brazil. They present phytochemical compounds with antioxidant and anti-inflammatory action. Therefore, the objective of this study was to analyze the antioxidant and anti-inflammatory activities of ethanolic extracts from leaves of and and its effect on the hepatic tissue in experimental knockout models after they received Paracetamol®.
Ethanol extracts from and leaves were prepared. Total phenolics were determined by Folin-Ciocalteau reagent, and flavonoids are based on the complexation reaction with the aluminum metal, forming a colored complex. Fingerprint HPLC was performed to detect phenolic compounds. Knockout IL-10 mice randomly divided into six groups were used and received the following treatments: G1, only water; G2, extract; G3, extract; G4, Paracetamol®; G5, Paracetamol® + extract (400 mg/kg); G6, Paracetamol® + extract (400 mg/kg). Biochemical parameters of the blood and differential count of leukocytes were done. Oxidative markers and histopathological analyses were performed on their liver tissue.
Phenolic compounds and total flavonoids were detected in both two extracts analyzed. The HPLC fingerprint detected phenolic acid, gallic acid, and catechin flavonoid in the two extracts. Histopathological analyses of the hepatic tissue permitted evaluation of nuclear increase, sinusoid congestion, and inflammatory infiltrate. pum presented more antioxidant activity increasing antioxidant enzyme levels and reducing TBARS and carbonyl protein when compared to the other treatments after exposure to Paracetamol®. Histopathological analyses showed a decrease in the inflammatory infiltrate after treatment with extracts.
Our findings indicate that both extracts, especially , can reduce hepatic damage in knockout mice exposed to paracetamol, indicating the curative power of these extracts reducing lipid peroxidation and in the morphofunctional damage to the liver parenchyma.
腰果树（L.，腰果）和卡朱伊树（D.）是巴西常见的植物。它们含有具有抗氧化和抗炎作用的植物化学化合物。因此，本研究的目的是分析腰果树和卡朱伊树叶片乙醇提取物的抗氧化和抗炎活性，以及它们对接受扑热息痛后的实验性基因敲除模型肝脏组织的影响。
制备了腰果树和卡朱伊树叶片的乙醇提取物。用福林-西奥尔特试剂测定总酚含量，黄酮类化合物基于与铝金属的络合反应，形成有色络合物。采用高效液相色谱指纹图谱法检测酚类化合物。使用随机分为六组的白细胞介素-10基因敲除小鼠，并给予以下处理：G1，仅饮水；G2，腰果树提取物；G3，卡朱伊树提取物；G4，扑热息痛；G5，扑热息痛+腰果树提取物（400mg/kg）；G6，扑热息痛+卡朱伊树提取物（400mg/kg）。进行了血液生化参数和白细胞分类计数。对其肝脏组织进行了氧化标记物和组织病理学分析。
在所分析的两种提取物中均检测到酚类化合物和总黄酮。高效液相色谱指纹图谱在两种提取物中检测到酚酸、没食子酸和儿茶素类黄酮。肝脏组织的组织病理学分析允许评估细胞核增多、窦状隙充血和炎症浸润。与扑热息痛处理后的其他处理相比，腰果树提取物表现出更强的抗氧化活性，增加了抗氧化酶水平，降低了丙二醛和羰基蛋白含量。组织病理学分析显示提取物处理后炎症浸润减少。
我们的研究结果表明，两种提取物，尤其是腰果树提取物，可减轻扑热息痛处理的基因敲除小鼠的肝脏损伤，表明这些提取物具有减轻脂质过氧化和对肝实质形态功能损伤的治疗作用。
