人降钙素基因相关肽激活腺苷酸环化酶并从人脐静脉内皮细胞释放前列环素。
Human calcitonin gene-related peptide activates adenylate cyclase and releases prostacyclin from human umbilical vein endothelial cells.
作者信息
Crossman D, McEwan J, MacDermot J, MacIntyre I, Dollery C T
机构信息
Department of Clinical Pharmacology, Royal Postgraduate Medical School, London.
出版信息
Br J Pharmacol. 1987 Dec;92(4):695-701. doi: 10.1111/j.1476-5381.1987.tb11373.x.
Abstract
1 Endothelial cells of human umbilical vein were isolated and cultured in vitro. 2 In these cells there was a concentration-dependent release of prostacyclin and activation of adenylate cyclase by human calcitonin gene-related peptide (hCGRP). The concentration of hCGRP for half-maximum activation of adenylate cyclase (Kact) by hCGRP was 190 nM. 3 Bradykinin induced a ten fold greater release of prostacyclin than CGRP, but did not activate adenylate cyclase. 4 hCGRP may exert its potent vasodilator properties by stimulating release of vasorelaxant substances, including prostacyclin from endothelial cells.
摘要
- 人脐静脉内皮细胞被分离并在体外培养。2. 在这些细胞中，人降钙素基因相关肽（hCGRP）可引起前列环素浓度依赖性释放及腺苷酸环化酶激活。hCGRP激活腺苷酸环化酶的半数最大激活浓度（Kact）为190 nM。3. 缓激肽诱导的前列环素释放比CGRP大10倍，但不激活腺苷酸环化酶。4. hCGRP可能通过刺激包括内皮细胞前列环素在内的血管舒张物质释放而发挥其强大的血管舒张特性。
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