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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Crossman D C, Dashwood M R, Brain S D, McEwan J, Pearson J D

Section of Vascular Biology, Clinical Research Centre, Harrow, Middlesex.

Br J Pharmacol. 1990 Jan;99(1):71-6. doi: 10.1111/j.1476-5381.1990.tb14656.x.

Bovine aortic endothelial cells (BAE) and smooth muscle cells (BASM) were grown separately and in co-culture. 2. Calcitonin gene-related peptide (CGRP) caused dose-dependent activation of adenylate cyclase in each cell type when grown in isolation. The concentration of CGRP causing half-maximal activation in BAE and BASM was 200 nM and 310 nM, respectively. 3. In cells grown in co-culture exposure to bradykinin produced dose-dependent elevations in cyclic GMP content which were maximal 1 min after application of the agonist. 4. CGRP (1 nM-1 microM) did not stimulate a rise in cyclic GMP in co-cultures. 5. Displaceable CGRP binding was identified throughout the wall of the bovine aorta. 6. We conclude that CGRP receptors coupled to adenylate cyclase are present on BAE and BASM, but there is no coupling of these receptors to the release of any agent (such as endothelium-derived relaxing factor) that activates guanylate cyclase.

分别培养牛主动脉内皮细胞（BAE）和平滑肌细胞（BASM），并进行共培养。2. 降钙素基因相关肽（CGRP）在单独培养时，能使每种细胞类型中的腺苷酸环化酶呈剂量依赖性激活。在BAE和BASM中引起半数最大激活的CGRP浓度分别为200 nM和310 nM。3. 在共培养的细胞中，暴露于缓激肽会使环鸟苷酸含量呈剂量依赖性升高，在应用激动剂后1分钟达到最大值。4. CGRP（1 nM - 1 microM）在共培养物中不会刺激环鸟苷酸升高。5. 在牛主动脉壁各处均鉴定出可置换的CGRP结合。6. 我们得出结论，与腺苷酸环化酶偶联的CGRP受体存在于BAE和BASM上，但这些受体与任何激活鸟苷酸环化酶的因子（如内皮衍生舒张因子）的释放无偶联关系。

Crossman D C, Dashwood M R, Brain S D, McEwan J, Pearson J D

Section of Vascular Biology, Clinical Research Centre, Harrow, Middlesex.

Br J Pharmacol. 1990 Jan;99(1):71-6. doi: 10.1111/j.1476-5381.1990.tb14656.x.

Bovine aortic endothelial cells (BAE) and smooth muscle cells (BASM) were grown separately and in co-culture. 2. Calcitonin gene-related peptide (CGRP) caused dose-dependent activation of adenylate cyclase in each cell type when grown in isolation. The concentration of CGRP causing half-maximal activation in BAE and BASM was 200 nM and 310 nM, respectively. 3. In cells grown in co-culture exposure to bradykinin produced dose-dependent elevations in cyclic GMP content which were maximal 1 min after application of the agonist. 4. CGRP (1 nM-1 microM) did not stimulate a rise in cyclic GMP in co-cultures. 5. Displaceable CGRP binding was identified throughout the wall of the bovine aorta. 6. We conclude that CGRP receptors coupled to adenylate cyclase are present on BAE and BASM, but there is no coupling of these receptors to the release of any agent (such as endothelium-derived relaxing factor) that activates guanylate cyclase.

分别培养牛主动脉内皮细胞（BAE）和平滑肌细胞（BASM），并进行共培养。2. 降钙素基因相关肽（CGRP）在单独培养时，能使每种细胞类型中的腺苷酸环化酶呈剂量依赖性激活。在BAE和BASM中引起半数最大激活的CGRP浓度分别为200 nM和310 nM。3. 在共培养的细胞中，暴露于缓激肽会使环鸟苷酸含量呈剂量依赖性升高，在应用激动剂后1分钟达到最大值。4. CGRP（1 nM - 1 microM）在共培养物中不会刺激环鸟苷酸升高。5. 在牛主动脉壁各处均鉴定出可置换的CGRP结合。6. 我们得出结论，与腺苷酸环化酶偶联的CGRP受体存在于BAE和BASM上，但这些受体与任何激活鸟苷酸环化酶的因子（如内皮衍生舒张因子）的释放无偶联关系。