Li Fuyong, Cheng Christopher C, Zheng Jinshui, Liu Junhong, Quevedo Rodrigo Margain, Li Junjie, Roos Stefan, Gänzle Michael G, Walter Jens
Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Alberta, T6G 2E1, Canada.
Department of Biological Sciences, University of Alberta, Edmonton, Alberta, T6G 2E1, Canada.
Int J Syst Evol Microbiol. 2021 Feb;71(2). doi: 10.1099/ijsem.0.004644.
Ten strains, BG-AF3-A, pH52_RY, WF-MT5-A, BG-MG3-A, Lr3000, RRLNB_1_1, STM3_1, STM2_1, WF-MO7-1 and WF-MA3-C, were isolated from intestinal or faecal samples of rodents, pheasant and primate. 16S rRNA gene analysis identified them as . However, average nucleotide identity and digital DNA-DNA hybridization values based on whole genomes were below 95 and 70 %, respectively, and thus below the threshold levels for bacterial species delineation. Based on genomic, chemotaxonomic and morphological analyses, we propose five novel species with the names sp. nov. (type strain BG-AF3-A=DSM 110574=LMG 31633), sp. nov. (type strain WF-MT5-A=DSM 110569=LMG 31629), sp. nov. (type strain Lr3000=DSM 110573=LMG 31632), sp. nov. (type strain STM3_1=DSM 110572=LMG 31631) and sp. nov. (type strain WF-MO7-1=DSM 110576=LMG 31630). Core genome phylogeny and experimental evidence of host adaptation of strains of further provide a strong rationale to consider a number of distinct lineages within this species as subspecies. Here we propose six subspecies of : subsp. subsp. nov. (type strain AP3=DSM 110703=LMG 31724), subsp. subsp. nov. (type strain 3c6=DSM 110571=LMG 31635), subsp. subsp. nov. (type strain lpuph1=DSM 110570=LMG 31634), subsp. subsp. nov. (type strain F 275=DSM 20016=ATCC 23272), subsp. subsp. nov. (type strain 1063=ATCC 53608=LMG 31752) and subsp. subsp. nov. (type strain 100-23=DSM 17509=CIP 109821).
从啮齿动物、雉鸡和灵长类动物的肠道或粪便样本中分离出了10个菌株，分别为BG-AF3-A、pH52_RY、WF-MT5-A、BG-MG3-A、Lr3000、RRLNB_1_1、STM3_1、STM2_1、WF-MO7-1和WF-MA3-C。16S rRNA基因分析将它们鉴定为 。然而，基于全基因组的平均核苷酸同一性和数字DNA-DNA杂交值分别低于95%和70%，因此低于细菌物种划分的阈值水平。基于基因组、化学分类学和形态学分析，我们提出了五个新物种，分别为 新种（模式菌株BG-AF3-A = DSM 110574 = LMG 31633）、 新种（模式菌株WF-MT5-A = DSM 110569 = LMG 31629）、 新种（模式菌株Lr3000 = DSM 110573 = LMG 31632）、 新种（模式菌株STM3_1 = DSM 110572 = LMG 31631）和 新种（模式菌株WF-MO7-1 = DSM 110576 = LMG 31630）。 菌株的核心基因组系统发育和宿主适应性的实验证据进一步提供了强有力的理由，可将该物种内的一些不同谱系视为亚种。在此，我们提出了 的六个亚种： 亚种 新亚种（模式菌株AP3 = DSM 110703 = LMG 31724）、 亚种 新亚种（模式菌株3c6 = DSM 110571 = LMG 31635）、 亚种 新亚种（模式菌株lpuph1 = DSM 110570 = LMG 31634）、 亚种 新亚种（模式菌株F 275 = DSM 20016 = ATCC 23272）、 亚种 新亚种（模式菌株1063 = ATCC 53608 = LMG 31752）和 亚种 新亚种（模式菌株100-23 = DSM 17509 = CIP 109821）。
