Tekeoğlu İbrahim, Akdoğan Mehmet, Çelik İlhami
Department of Rheumatology/Rehabilitation, Research and Teaching Hospital, Sakarya University, Turkey.
Department of Medical Biochemistry, Medical Faculty, Sakarya University, Turkey.
Reumatologia. 2020;58(5):265-271. doi: 10.5114/reum.2020.99764. Epub 2020 Oct 15.
Rheumatoid arthritis is a multisystemic inflammatory disease characterized by destruction of the joints. An effective treatment method of the disease has not been developed yet. The aim of the present study is to evaluate the effects of bee () venom (BV) on serum inflammatory parameters, serum antioxidant load and clinical parameters of experimentally induced adjuvant arthritis in rats.
A total of 35 Wistar albino male rats were used. The animals were divided into 5 groups. First group animals served as negative controls. The second, third, fourth and fifth groups were used for experimental arthritis induction. Following clinical development of arthritis, the first group was subcutaneously administered 0.2 ml of physiological saline, and the second, third and fourth groups were treated subcutaneously with 2 µg/kg, 4 µg/kg and 20 µg/kg once a week three times. Physiological saline injected fifth group animals were used as a sham-treatment group. Clinical observations and evaluation of arthritis were made at the 15 day, and at the end of the experiment. The levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase, paraoxonase, serum aryl esterase, high-sensitivity C reactive protein, interleukin 1β (IL-1β), interleukin 6 (IL-6), and tumor necrosis factor alpha (TNF-α) were determined in cardiac blood samples taken at the end of the 29 day.
From the data, total oxidant level (TOL) and oxidative stress index (OSI) were calculated. Significant improvements were observed in the clinical signs of arthritis and inflammatory markers such as in IL-1β, TNF-α, IL-6 and TOL and OSI in the 20.0 µg/kg BV-administered group. Bee venom administration did not cause any significant increase in ALT and AST values or signs of liver toxicity.
Bee venom treatment was effective in alleviation of symptoms of the experimental rat adjuvant arthritis by means of clinical observation and serum inflammatory markers.
类风湿性关节炎是一种以关节破坏为特征的多系统性炎症性疾病。目前尚未开发出有效的治疗方法。本研究的目的是评估蜂毒对实验性诱导的大鼠佐剂性关节炎的血清炎症参数、血清抗氧化负荷和临床参数的影响。
总共使用了35只雄性Wistar白化大鼠。将动物分为5组。第一组动物作为阴性对照。第二、三、四和五组用于实验性关节炎诱导。关节炎临床症状出现后，第一组皮下注射0.2 ml生理盐水，第二、三、四组每周皮下注射一次，剂量分别为2 μg/kg、4 μg/kg和20 μg/kg，共注射三次。注射生理盐水的第五组动物用作假治疗组。在第15天和实验结束时进行关节炎的临床观察和评估。在第29天结束时采集心脏血样，测定天冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）、碱性磷酸酶、对氧磷酶、血清芳基酯酶、高敏C反应蛋白、白细胞介素1β（IL-1β）、白细胞介素6（IL-6）和肿瘤坏死因子α（TNF-α）的水平。
根据数据计算了总氧化剂水平（TOL）和氧化应激指数（OSI）。在给予20.0 μg/kg蜂毒的组中，观察到关节炎临床症状和炎症标志物如IL-1β、TNF-α、IL-6以及TOL和OSI有显著改善。蜂毒给药未导致ALT和AST值显著升高或出现肝毒性迹象。
通过临床观察和血清炎症标志物发现，蜂毒治疗对缓解实验性大鼠佐剂性关节炎症状有效。
