Wang Ao, Wang Rui, Miao Xiaoling
State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.
Carbon-Negative Synthetic Biology for Biomaterial Production from CO2 (CNSB), Campus for Research Excellence and Technological Enterprise (CREATE), 1 CREATE Way, Singapore 138602, Singapore.
Int J Mol Sci. 2025 Jan 17;26(2):769. doi: 10.3390/ijms26020769.
Alkaline environments such as alkaline lands, lakes, and industrial wastewater are not conducive to the growth of plants and microorganisms due to high pH and salinity. ChbZIP1 is a bZIP family transcription factor isolated from an alkaliphilic microalgae ( sp. BLD). Previous studies have demonstrated its ability to enhance alkaline tolerance in . However, the potential of ChbZIP1 to confer similar alkaline tolerance in other microalgae remains unclear, and the specific mechanisms are not fully understood. The analysis of cellular physiological and biochemical indicators revealed that the ChbZIP1 transformants exhibited enhanced photosynthetic activity, increased lipid accumulation, and reduced fatty acid unsaturation. Genes associated with cellular reactive oxygen species (ROS) detoxification were found to be upregulated, and a corresponding increase in antioxidant enzyme activity was detected. In addition, the relative abundance of intracellular ROS and malondialdehyde (MDA) was significantly lower in the transformants. In summary, our research indicates that ChbZIP1 enhances the tolerance of to alkaline environments through several mechanisms, including the repair of damaged photosynthesis, increased lipid accumulation, improved fatty acid unsaturation, and enhanced antioxidant enzyme activity. This study aims to contribute to a more comprehensive understanding of the mechanisms underlying alkalinity tolerance in microalgae and offers new insights and theoretical foundations for the utilization of microalgae in alkaline environments.
诸如盐碱地、湖泊和工业废水等碱性环境，由于pH值高和盐度大，不利于植物和微生物的生长。ChbZIP1是一种从嗜碱微藻（sp. BLD）中分离出来的bZIP家族转录因子。先前的研究已经证明了它在[具体对象未明确]中增强耐碱性的能力。然而，ChbZIP1在其他微藻中赋予类似耐碱性的潜力仍不清楚，具体机制也尚未完全了解。对细胞生理和生化指标的分析表明，ChbZIP1转化体表现出增强的光合活性、增加的脂质积累和降低的脂肪酸不饱和度。发现与细胞活性氧（ROS）解毒相关的基因上调，并且检测到抗氧化酶活性相应增加。此外，转化体中细胞内ROS和丙二醛（MDA）的相对丰度显著降低。总之，我们的研究表明，ChbZIP1通过多种机制增强[具体对象未明确]对碱性环境的耐受性，包括修复受损的光合作用、增加脂质积累、改善脂肪酸不饱和度和增强抗氧化酶活性。本研究旨在有助于更全面地理解微藻耐碱性的潜在机制，并为微藻在碱性环境中的利用提供新的见解和理论基础。
