Studies in Human Life Sciences, Graduate School of Studies in Human Culture, Otsuma Women's University, Chiyoda-ku, Tokyo 102-8357, Japan.
Research and Development Department, Hakubaku Co. Ltd., Chuo-City, Yamanashi 409-3843, Japan.
Nutrients. 2020 Nov 19;12(11):3546. doi: 10.3390/nu12113546.
We evaluated whether intake of β-glucan-rich barley flour affects expression levels of genes related to glucose and lipid metabolism in the ileum, liver, and adipose tissues of mice fed a high-fat diet. C57BL/6J male mice were fed a high-fat diet supplemented with high β-glucan barley, for 92 days. We measured the expression levels of genes involved in glucose and lipid metabolism in the ileum, liver, and adipose tissues using DNA microarray and q-PCR. The concentration of short-chain fatty acids (SCFAs) in the cecum was analyzed by GC/MS. The metabolic syndrome indices were improved by barley flour intake. Microarray analysis showed that the expression of genes related to steroid synthesis was consistently decreased in the liver and adipose tissues. The expression of genes involved in glucose metabolism did not change in these organs. In liver, a negative correlation was showed between some SCFAs and the expression levels of mRNA related to lipid synthesis and degradation. Barley flour affects lipid metabolism at the gene expression levels in both liver and adipose tissues. We suggest that SCFAs are associated with changes in the expression levels of genes related to lipid metabolism in the liver and adipose tissues, which affect lipid accumulation.
我们评估了富含β-葡聚糖的大麦粉的摄入是否会影响高脂肪饮食喂养的小鼠回肠、肝脏和脂肪组织中与葡萄糖和脂质代谢相关的基因的表达水平。C57BL/6J 雄性小鼠用高脂肪饲料加富含β-葡聚糖的大麦喂养 92 天。我们使用 DNA 微阵列和 q-PCR 测量了回肠、肝脏和脂肪组织中参与葡萄糖和脂质代谢的基因的表达水平。通过 GC/MS 分析了盲肠中短链脂肪酸（SCFAs）的浓度。大麦粉的摄入改善了代谢综合征指数。微阵列分析表明，肝脏和脂肪组织中与类固醇合成有关的基因表达持续下降。这些器官中葡萄糖代谢相关基因的表达没有改变。在肝脏中，一些 SCFAs 与与脂质合成和降解相关的 mRNA 表达水平之间呈负相关。大麦粉在肝脏和脂肪组织中均影响脂质代谢的基因表达水平。我们认为 SCFAs 与肝脏和脂肪组织中与脂质代谢相关的基因表达水平的变化有关，这会影响脂质的积累。
