Mio Kento, Otake Nami, Nakashima Satoko, Matsuoka Tsubasa, Aoe Seiichiro
Studies in Human Life Sciences, Graduate School of Studies in Human Culture, Otsuma Women's University, Chiyoda-ku, Tokyo 102-8357, Japan.
Research and Development Department, Hakubaku Co. Ltd., Chuo-City, Yamanashi 409-3843, Japan.
Nutrients. 2021 Mar 11;13(3):907. doi: 10.3390/nu13030907.
The prebiotic effect of high β-glucan barley (HGB) flour on the innate immune system of high-fat model mice was investigated. C57BL/6J male mice were fed a high-fat diet supplemented with HGB flour for 90 days. Secretory immunoglobulin A (sIgA) in the cecum and serum were analyzed by enzyme-linked immunosorbent assays (ELISA). Real-time PCR was used to determine mRNA expression levels of pro- and anti-inflammatory cytokines such as interleukin (IL)-10 and IL-6 in the ileum as well as the composition of the microbiota in the cecum. Concentrations of short-chain fatty acids (SCFAs) and organic acids were analyzed by GC/MS. Concentrations of sIgA in the cecum and serum were increased in the HGB group compared to the control. Gene expression levels of and polymeric immunoglobulin receptor () significantly increased in the HGB group. HGB intake increased the bacterial count of microbiota, such as and . Concentrations of propionate and lactate in the cecum were increased in the HGB group, and a positive correlation was found between these organic acids and the expression level. Our findings showed that HGB flour enhanced immune function such as IgA secretion and expression, even when the immune system was deteriorated by a high-fat diet. Moreover, we found that HGB flour modulated the gut microbiota, which increased the concentration of SCFAs, thereby stimulating the immune system.
研究了高β-葡聚糖大麦（HGB）粉对高脂模型小鼠先天免疫系统的益生元效应。将C57BL/6J雄性小鼠喂食添加了HGB粉的高脂饮食90天。通过酶联免疫吸附测定（ELISA）分析盲肠和血清中的分泌型免疫球蛋白A（sIgA）。使用实时PCR测定回肠中促炎和抗炎细胞因子如白细胞介素（IL）-10和IL-6的mRNA表达水平以及盲肠中微生物群的组成。通过GC/MS分析短链脂肪酸（SCFA）和有机酸的浓度。与对照组相比，HGB组盲肠和血清中的sIgA浓度增加。HGB组中 和多聚免疫球蛋白受体（ ）的基因表达水平显著增加。摄入HGB增加了微生物群的细菌数量，如 和 。HGB组盲肠中丙酸和乳酸的浓度增加，并且发现这些有机酸与 表达水平之间存在正相关。我们的研究结果表明，即使在高脂饮食使免疫系统恶化的情况下，HGB粉也能增强免疫功能，如IgA分泌和 表达。此外，我们发现HGB粉调节肠道微生物群，增加了SCFA的浓度，从而刺激了免疫系统。
