Kamfwa Kelvin, Cichy Karen A, Kelly James D
Dep. of Plant, Soil and Microbial Sciences, Michigan State Univ., 1066 Bogue St., East Lansing, MI, 48824.
USDA-ARS, Sugarbeet and Bean Research Unit, Michigan State Univ., 1066 Bogue St., East Lansing, MI, 48824.
Plant Genome. 2015 Jul;8(2):eplantgenome2014.09.0059. doi: 10.3835/plantgenome2014.09.0059.
A genome-wide association study (GWAS) using a global Andean diversity panel (ADP) of 237 genotypes of common bean (Phaseolus vulgaris L.) was conducted to gain insight into the genetic architecture of phenology, biomass, yield components, and seed yield traits. The panel was evaluated for 2 yr in field trials in Michigan and genotyped with 5398 single nucleotide polymorphism (SNP) markers. After correcting for population structure and cryptic relatedness, significant SNP markers associated with several agronomic traits were identified. Positional candidate genes, including Phvul.001G221100 on P. vulgaris (Pv) chromosome 01, associated with days to flowering and maturity were identified. Significant SNPs for seed yield were identified on Pv03 and Pv09 and colocalized with quantitative trait loci (QTL) for yield from previous studies conducted in several environments and contrasting genetic backgrounds. The majority of germplasm carrying the alleles with positive effects on seed yield was of African origin and largely underutilized in US breeding programs. The study provided insights into the genetic architecture of agronomic traits in Andean beans.
利用一个包含237个普通菜豆（Phaseolus vulgaris L.）基因型的全球安第斯多样性面板（ADP）进行了全基因组关联研究（GWAS），以深入了解物候、生物量、产量构成因素和种子产量性状的遗传结构。该面板在密歇根州的田间试验中进行了两年评估，并用5398个单核苷酸多态性（SNP）标记进行了基因分型。在对群体结构和潜在相关性进行校正后，鉴定出了与多个农艺性状相关的显著SNP标记。确定了与开花天数和成熟天数相关的定位候选基因，包括普通菜豆（Pv）01号染色体上的Phvul.001G221100。在Pv03和Pv09上鉴定出了种子产量的显著SNP，并且与之前在多个环境和不同遗传背景下进行的研究中产量的数量性状位点（QTL）共定位。大多数携带对种子产量有积极影响等位基因的种质起源于非洲，在美国育种计划中基本上未得到充分利用。该研究为安第斯菜豆农艺性状的遗传结构提供了见解。
