Department of Pharmaceutical Engineering, College of Materials and Energy, State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, Guangdong Province Research Center of Woody Forage Engineering Technology, South China Agricultural University, Guangzhou 510642, PR China.
Department of Immunology and Infectious Diseases, John Curtin School of Medical Research, The Australian National University, Canberra, Australia.
Int J Biol Macromol. 2019 Jul 1;132:844-851. doi: 10.1016/j.ijbiomac.2019.03.210. Epub 2019 Mar 29.
Moringa oleifera is a mutli-purpose herbal plant which has attained enormous attention as a natural source of nutrients and folk medicine. This work aimed to get a novel polysaccharide, termed MRP-1, which was isolated from Moringa oleifera roots with hot water extraction method followed by ethanol precipitation and purified with DEAE-Sepharose Fast Flow column. Monosaccharide composition analysis based on GC-MS showed that MRP-1 mainly consisted of Rha, Ara, Fru, Xyl, Man and Gal in the molar ratio of 1.5:2.0:3.1:6.0:5.3:1.1. The Roman spectra, FT-IR and NMR analysis showed that the typical features of carbohydrates, such as α-Araf, α-Gly, β-Galp, α-GalpA and β-Gly was contained by MRP-1. The LPS-induced RAW264.7 macrophage cells were used to evaluate the anti-inflammatory activity of MRP-1. The result demonstrated that the increasing of NO and TNF-α production induced by LPS could be prevented by different concentrations of MRP-1 treatment. Moreover, the mRNA expression level of iNOS induced by LPS was decreased significantly (p < 0.05) by MRP-1 treatment while show no obvious effect on the COX-2 mRNA expression. This study may provide new possible application of Moringa oleifera root polysaccharide related to anti-inflammation.
辣木是一种多用途的草本植物，作为营养物质的天然来源和民间药物而备受关注。本工作旨在从辣木根中分离出一种新型多糖，称为 MRP-1，采用热水提取法提取，乙醇沉淀，再用 DEAE-Sepharose Fast Flow 柱纯化。基于 GC-MS 的单糖组成分析表明，MRP-1 主要由 Rha、Ara、Fru、Xyl、Man 和 Gal 组成，摩尔比为 1.5:2.0:3.1:6.0:5.3:1.1。罗马光谱、FT-IR 和 NMR 分析表明，MRP-1 含有碳水化合物的典型特征，如α-Araf、α-Gly、β-Galp、α-GalpA 和β-Gly。采用 LPS 诱导的 RAW264.7 巨噬细胞评估 MRP-1 的抗炎活性。结果表明，不同浓度的 MRP-1 处理可防止 LPS 诱导的 NO 和 TNF-α 产生增加。此外，MRP-1 处理可显著降低 LPS 诱导的 iNOS mRNA 表达水平（p<0.05），而对 COX-2 mRNA 表达无明显影响。这项研究可能为辣木根多糖的抗炎新应用提供了可能。
