Hammerschlag M R, Roblin P M, Cummings C, Williams T H, Worku M, Howard L V
Department of Pediatrics, State University of New York, Brooklyn 11203.
J Clin Microbiol. 1987 Dec;25(12):2306-8. doi: 10.1128/jcm.25.12.2306-2308.1987.
The efficacy of Chlamydiazyme (Abbott Laboratories, North Chicago, Ill.) in detecting neonatal conjunctival and respiratory infections caused by Chlamydia trachomatis was determined by comparison of this enzyme immunoassay (EIA) with the method of isolation of chlamydiae in tissue culture. The sensitivity and specificity of Chlamydiazyme for detecting C. trachomatis in conjunctival specimens from infants with conjunctivitis were 98 and 94%, respectively. For nasopharyngeal infection in infants with conjunctivitis, the sensitivity and specificity were 87 and 92%, respectively. There were nine nasopharyngeal specimens that were Chlamydiazyme positive and culture negative. All of these specimens demonstrated the presence of typical fluorescing chlamydial elementary bodies when pellets of the original specimens were examined with a fluorescein-conjugated monoclonal antibody. When the EIA was performed on nasopharyngeal specimens from infants with suspected chlamydial pneumonia, 6 culture-positive and 10 culture-negative specimens were correctly identified.
通过将衣原体酶免疫测定法（EIA）（Chlamydiazyme，雅培实验室，伊利诺伊州北芝加哥）与衣原体在组织培养中的分离方法进行比较，来确定其在检测沙眼衣原体引起的新生儿结膜和呼吸道感染方面的功效。对于结膜炎患儿结膜标本中沙眼衣原体的检测，衣原体酶的敏感性和特异性分别为98%和94%。对于结膜炎患儿的鼻咽部感染，敏感性和特异性分别为87%和92%。有9份鼻咽标本衣原体酶检测呈阳性但培养阴性。当用荧光素偶联单克隆抗体检查原始标本沉淀时，所有这些标本均显示存在典型的荧光衣原体原体。当对疑似衣原体肺炎患儿的鼻咽标本进行EIA检测时，6份培养阳性和10份培养阴性的标本被正确鉴定。
