人乳腺癌冷冻切片雌激素受体测定及同步定量组织学分析
Oestrogen receptor assay of cryostat sections of human breast carcinomas with simultaneous quantitative histology.
作者信息
Underwood J C, Dangerfield V J, Parsons M A
出版信息
J Clin Pathol. 1983 Apr;36(4):399-405. doi: 10.1136/jcp.36.4.399.
Abstract
Cryostat sections of unfixed human breast carcinomas were assayed for oestrogen receptor (ER) content using an isoelectric focusing method to separate the receptor-bound oestradiol. Adjacent sections from the same tissue block were stained so that the tumour content could be estimated by point counting and the ER concentration adjusted to compensate for variations in cellularity. Elastosis was also assessed. The results confirm a positive correlation between ER values and both cellularity and elastosis. The measurement of ER in cryostat sections is relatively simple and rapid, is applicable to small tissue samples, and permits histological identification of the nature and composition of the assayed sample. The method is directly applicable to oestrogen receptor analyses of breast carcinomas in clinical laboratories with facilities for cryostat microtomy.
摘要
使用等电聚焦法分离受体结合的雌二醇，对未固定的人乳腺癌冰冻切片进行雌激素受体（ER）含量检测。对来自同一组织块的相邻切片进行染色，以便通过点计数估计肿瘤含量，并调整ER浓度以补偿细胞密度的变化。同时也评估了弹性组织变性。结果证实ER值与细胞密度和弹性组织变性之间呈正相关。在冰冻切片中测量ER相对简单、快速，适用于小组织样本，并允许对检测样本的性质和组成进行组织学鉴定。该方法可直接应用于具备冰冻切片机的临床实验室对乳腺癌进行雌激素受体分析。
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