Giriwono Puspo Edi, Iskandriati Diah, Tan Chin Ping, Andarwulan Nuri
Department of Food Science and Technology, Faculty of Agricultural Engineering and Technology, Bogor Agricultural University 16680, Indonesia.
Department of Food Science and Technology, Faculty of Agricultural Engineering and Technology, Bogor Agricultural University 16680, Indonesia; Southeast Asian Food and Agricultural Science Technology (SEAFAST) Center, Bogor Agricultural University 16680, Indonesia.
Food Res Int. 2020 Nov;137:109702. doi: 10.1016/j.foodres.2020.109702. Epub 2020 Sep 18.
Sargassum brown seaweed is well-known to contain several bioactive compounds which exhibit various biological activities, including anti-inflammatory and antioxidant activity. Lipophilic extracts and fractions of Sargassum were reported to possess promising anti-inflammatory activity. This study, therefore, aims to evaluate the anti-inflammatory and antioxidant activity of Sargassum cristaefolium crude lipid extract and its fractions. The brown seaweed was obtained from Awur Bay, Jepara - Indonesia. Crude lipid fractionation was performed using normal phase column chromatography, and three different fractions (dichloromethane, acetone, methanol) were produced. The results showed that treatment of acetone fraction exerted strongest nitric oxide inhibition in lipopolysaccharide-induced RAW 264.7 cells, both in pre-incubated and co-incubated cell culture models. This outcome was in accordance with its 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity and ferric reducing antioxidant power (FRAP). Metabolite profiling of lipid fractions was performed by ultra-high-performance liquid chromatography electrospray ionization orbitrap tandem mass spectrometry, while the orthogonal projection to latent structures analysis was conducted to determine some features with significant correlation to the bioactivity. There were 14 feature candidates considered from both positive and negative ionization mode datasets. Seven out of them were putatively identified as pheophytin a (1), all-trans fucoxanthin (2), 13-hydroxy-pheophytin a (3), pheophorbide a (4), 1-hexadecanoyl-2-(9Z-octadecenoyl)-3-O-β-D-galactosyl-sn-glycerol (6), 1-(5Z,8Z,11Z,14Z,17Z-eicosapentaenoyl)-2-(9Z,12Z,15Z-octadecatrienoyl)-3-O-β-D-galactosyl-sn-glycerol (10), and 1-(9Z,12Z,15Z-octadecatrienoyl)-2-(6Z,9Z,12Z,15Z-octadecatetraenoyl)-3-O-β-D-galactosyl-sn glycerol (12).
马尾藻褐藻因含有多种具有多种生物活性的生物活性化合物而闻名，这些活性包括抗炎和抗氧化活性。据报道，马尾藻的亲脂性提取物和馏分具有良好的抗炎活性。因此，本研究旨在评估波状马尾藻粗脂质提取物及其馏分的抗炎和抗氧化活性。这种褐藻取自印度尼西亚杰帕拉的阿武尔湾。使用正相柱色谱法进行粗脂质分级分离，得到三种不同的馏分（二氯甲烷、丙酮、甲醇）。结果表明，在脂多糖诱导的RAW 264.7细胞中，无论是预孵育还是共孵育细胞培养模型，丙酮馏分处理对一氧化氮的抑制作用最强。这一结果与其对2,2-二苯基-1-苦基肼（DPPH）的清除活性和铁还原抗氧化能力（FRAP）一致。通过超高效液相色谱电喷雾电离轨道阱串联质谱对脂质馏分进行代谢物谱分析，同时进行正交投影到潜在结构分析以确定与生物活性有显著相关性的一些特征。从正离子和负离子电离模式数据集中共考虑了14个特征候选物。其中7种被推定鉴定为脱镁叶绿素a（1）、全反式岩藻黄质（2）、13-羟基脱镁叶绿素a（3）、脱镁叶绿酸a（4）、1-十六烷酰基-2-（9Z-十八碳烯酰基）-3-O-β-D-半乳糖基-sn-甘油（6）、1-（5Z,8Z,11Z,14Z,17Z-二十碳五烯酰基）-2-（9Z,12Z,15Z-十八碳三烯酰基）-3-O-β-D-半乳糖基-sn-甘油（10）和1-（9Z,12Z,15Z-十八碳三烯酰基）-2-（6Z,9Z,12Z,15Z-十八碳四烯酰基）-3-O-β-D-半乳糖基-sn甘油（12）。
