Department of Microscopic Morphology/Histology, Angiogenesis Research Center, Faculty of Medicine, "Victor Babes" University of Medicine and Pharmacy, 2 Eftimie Murgu Square, 300041 Timisoara, Romania.
Department of Pharmaceutical Physics, Faculty of Pharmacy, "Victor Babes" University of Medicine and Pharmacy, 2 Eftimie Murgu Square, 300041 Timisoara, Romania.
Molecules. 2020 Nov 20;25(22):5442. doi: 10.3390/molecules25225442.
The current study was aimed to evaluate the phenolic composition parameters of two hydro-alcoholic extracts of L. (OB) obtained from the aerial part (without leaves) and leaves, in order to determine their contribution to the antioxidant activity (AOA). Both hydro-alcoholic extracts have proven to be rich in polyphenolic compounds, flavonoids, flavonols and tannins. Therefore, the leaves' extracts reveal an inhibition percentage of 89%, almost comparable with the standard reference (95%). To complete the toxicological profile, the study also assessed the potential cytotoxicity of basil hydro-alcoholic extracts on immortalized human keratinocytes (HaCaT), skin human fibroblasts (1BR3), mice epidermis (JB6Cl41-5a) and primary human melanocytes (HEMa) cells, correlated to A375 antitumor in vitro activity. The extracts did not induce significant cytotoxic effect on any of the selected normal cell lines but showed relevant activity on A375 cells. Considering the low values obtained regarding the irritative effects in the chorionallantoic membrane of the egg on blood vessels, we can emphasize that both extracts can be considered as biocompatible ingredients. Regarding the potential activity of hydro-alcoholic extracts on human skin, the decrease of erythema values after the application of extracts was a relevant observation which indicates the anti-inflammatory potential of L.
本研究旨在评估两种来自 （OB）地上部分（无叶）和叶部的水醇提取物的酚类成分参数，以确定它们对抗氧化活性（AOA）的贡献。两种水醇提取物均富含多酚类化合物、类黄酮、黄酮醇和单宁。因此，叶部提取物的抑制率达到 89%，几乎可与标准参考物（95%）相媲美。为了完善毒理学特征，该研究还评估了罗勒水醇提取物对永生化人角质形成细胞（HaCaT）、人皮肤成纤维细胞（1BR3）、小鼠表皮（JB6Cl41-5a）和原代人黑素细胞（HEMa）的潜在细胞毒性，这与 A375 的体外抗肿瘤活性相关。提取物对所选的任何正常细胞系均未引起明显的细胞毒性作用，但对 A375 细胞表现出相关活性。考虑到在鸡胚绒毛尿囊膜上对血管的刺激性效应中获得的低数值，我们可以强调这两种提取物均可被视为生物相容性成分。关于水醇提取物对人类皮肤的潜在活性，在应用提取物后红斑值的降低是一个重要的观察结果，这表明 对炎症的潜在抑制作用。
