Avignon Univ, INRAE, UMR SQPOV, SporAlim Team, Domaine Saint-Paul-Site Agroparc, 84914 Avignon CEDEX 9, France.
Aix-Marseille Univ, INSERM, SSA, IRBA, MCT, UMR_MD1, U-1261, 13005 Marseille CEDEX 5, France.
Molecules. 2020 Nov 20;25(22):5448. doi: 10.3390/molecules25225448.
Today, an alarming rise of bacterial gastroenteritis in humans resulting from consuming -tainted foods is being observed. One of the solutions for mitigating this issue may be the antibacterial activity of essential oils. In the present research, we propose to study the antibacterial activity against and other Gram-negative bacteria of essential oil and its active molecules. In addition, a few chemically synthesized molecules such as ()-methylisoeugenol, Elemicin, and eugenol were also studied. The results showed that the essential oil itself and its most active component, ()-methylisoeugenol, exhibited bactericidal effects. Similar effects were detected using purified and chemically synthesized molecules. Also, it was observed that the essential oil and its active molecules affected intracellular potassium and intracellular ATP contents in cells. Inhibition of the membrane bound FF-ATPase was also observed. Eventually, for the first time, the efflux mechanism of active molecules of essential oil was also identified in gamma proteobacteria and its specific antibacterial activity against was associated with the lack of this efflux mechanism in this species.
如今，人们观察到食用受污染食物导致人类细菌性肠胃炎的发病率急剧上升。解决这个问题的方法之一可能是精油的抗菌活性。在本研究中，我们建议研究精油及其活性分子对 和其他革兰氏阴性菌的抗菌活性。此外，还研究了一些化学合成的分子，如（）-甲基异丁香酚、艾里莫芬辛和丁香酚。结果表明，精油本身及其最活跃的成分（）-甲基异丁香酚具有杀菌作用。使用纯化和化学合成的分子也检测到了类似的效果。此外，还观察到 精油及其活性分子影响 细胞内钾和细胞内 ATP 含量。还观察到膜结合 FF-ATPase 的抑制。最后，首次鉴定了 精油的活性分子在γ变形菌中的外排机制，并且其针对 的特殊抗菌活性与该物种中缺乏这种外排机制有关。
