Sam Ann Susan, Ninan Marilyn Mary, Ranjani R, Devanga Raghupathi Naveen Kumar, Balaji Veeraraghavan, Michael Joy Sarojini
Department of Clinical Microbiology, Christian Medical College, Vellore-632004, India.
Future Sci OA. 2020 Jul 30;6(9):FSO612. doi: 10.2144/fsoa-2020-0023.
To evaluate the performance of VITEKMS with DNA sequencing for laboratory diagnosis of non-tuberculous mycobacteria (NTM) species in a resource-limited setting.
sequencing and MALDI-TOF mass spectrometry (VITEKMS) was performed at a tertiary-care hospital in India. MALDI-TOF results were confirmed by 16S rRNA sequencing. In addition, sequencing of the internal transcribed spacer region was performed on slowly growing NTM.
Commonest species isolated were , and . sequencing and MALDI-TOF results had agreement of 94.5% for rapidly growing and 77.5% for slowly growing NTM.
There is good correlation between VITEKMS and sequencing for rapidly growing NTM. For slowly growing species, sequencing would be required in a third isolates.
评估在资源有限的环境中，VITEK MS与DNA测序技术用于非结核分枝杆菌（NTM）菌种实验室诊断的性能。
在印度一家三级医疗医院进行测序和基质辅助激光解吸电离飞行时间质谱分析（VITEK MS）。MALDI-TOF结果通过16S rRNA测序进行确认。此外，对生长缓慢的NTM进行内部转录间隔区测序。
分离出的最常见菌种为 、 和 。对于快速生长的NTM，测序和MALDI-TOF结果的一致性为94.5%，对于生长缓慢的NTM为77.5%。
对于快速生长的NTM，VITEK MS与测序之间存在良好的相关性。对于生长缓慢的菌种，三分之一的分离株需要进行测序。
