• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

定量分析组蛋白特定位置乙酰化对染色质转录速率的影响。

Quantification of the effect of site-specific histone acetylation on chromatin transcription rate.

机构信息

Laboratory for Epigenetics Drug Discovery, RIKEN Center for Biosystems Dynamics Research, Yokohama, Kanagawa 230-0045, Japan.

Graduate School of Pharmaceutical Sciences, The University of Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan.

出版信息

Nucleic Acids Res. 2020 Dec 16;48(22):12648-12659. doi: 10.1093/nar/gkaa1050.

DOI:10.1093/nar/gkaa1050
PMID:33238306
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7736822/
Abstract

Eukaryotic transcription is epigenetically regulated by chromatin structure and post-translational modifications (PTMs). For example, lysine acetylation in histone H4 is correlated with activation of RNA polymerase I-, II- and III-driven transcription from chromatin templates, which requires prior chromatin remodeling. However, quantitative understanding of the contribution of particular PTM states to the sequential steps of eukaryotic transcription has been hampered partially because reconstitution of a chromatin template with designed PTMs is difficult. In this study, we reconstituted a di-nucleosome with site-specifically acetylated or unmodified histone H4, which contained two copies of the Xenopus somatic 5S rRNA gene with addition of a unique sequence detectable by hybridization-assisted fluorescence correlation spectroscopy. Using a Xenopus oocyte nuclear extract, we analyzed the time course of accumulation of nascent 5S rRNA-derived transcripts generated on chromatin templates in vitro. Our mathematically described kinetic model and fitting analysis revealed that tetra-acetylation of histone H4 at K5/K8/K12/K16 increases the rate of transcriptionally competent chromatin formation ∼3-fold in comparison with the absence of acetylation. We provide a kinetic model for quantitative evaluation of the contribution of epigenetic modifications to chromatin transcription.

摘要

真核生物转录受染色质结构和翻译后修饰（PTMs）的表观遗传调控。例如，组蛋白 H4 中的赖氨酸乙酰化与 RNA 聚合酶 I、II 和 III 驱动的从染色质模板转录的激活相关，这需要预先进行染色质重塑。然而，由于用设计的 PTM 重建染色质模板具有一定的难度，因此，对特定 PTM 状态对真核转录的连续步骤的贡献的定量理解受到了部分阻碍。在这项研究中，我们使用杂交辅助荧光相关光谱法可检测到的独特序列，在含有两个 Xenopus 体细胞 5S rRNA 基因拷贝的二核小体上重新构建了具有特异性乙酰化或未修饰组蛋白 H4 的二核小体。使用 Xenopus 卵母细胞核提取物，我们分析了体外在染色质模板上生成的新生 5S rRNA 衍生转录物的积累的时程。我们描述的数学模型和拟合分析表明，与没有乙酰化相比，组蛋白 H4 的 K5/K8/K12/K16 四乙酰化将转录活性染色质形成的速率提高了约 3 倍。我们提供了一个用于定量评估表观遗传修饰对染色质转录贡献的动力学模型。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a157/7736822/c7719661eba7/gkaa1050fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a157/7736822/7a18a29e8dfa/gkaa1050fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a157/7736822/3f3708b91a4a/gkaa1050fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a157/7736822/f2073757da4b/gkaa1050fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a157/7736822/4928561db928/gkaa1050fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a157/7736822/c7719661eba7/gkaa1050fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a157/7736822/7a18a29e8dfa/gkaa1050fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a157/7736822/3f3708b91a4a/gkaa1050fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a157/7736822/f2073757da4b/gkaa1050fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a157/7736822/4928561db928/gkaa1050fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a157/7736822/c7719661eba7/gkaa1050fig5.jpg

相似文献

1
Quantification of the effect of site-specific histone acetylation on chromatin transcription rate.定量分析组蛋白特定位置乙酰化对染色质转录速率的影响。
Nucleic Acids Res. 2020 Dec 16;48(22):12648-12659. doi: 10.1093/nar/gkaa1050.
2
Histone H1 binding does not inhibit transcription of nucleosomal Xenopus laevis somatic 5S rRNA templates.组蛋白H1的结合并不抑制核小体非洲爪蟾体细胞5S rRNA模板的转录。
Biochemistry. 1998 May 19;37(20):7077-82. doi: 10.1021/bi980410o.
3
Both the 5S rRNA gene and the AT-rich flanks of xenopus laevis oocyte-type 5S rDNA repeat are required for histone H1-dependent repression of transcription of pol III-type genes in in vitro reconstituted chromatin.非洲爪蟾卵母细胞型5S rDNA重复序列的5S rRNA基因和富含AT的侧翼序列对于体外重构染色质中组蛋白H1依赖的pol III型基因转录抑制都是必需的。
Nucleic Acids Res. 1998 Dec 15;26(24):5596-601. doi: 10.1093/nar/26.24.5596.
4
Transcriptionally active Xenopus laevis somatic 5 S ribosomal RNA genes are packaged with hyperacetylated histone H4, whereas transcriptionally silent oocyte genes are not.转录活跃的非洲爪蟾体细胞5S核糖体RNA基因与高度乙酰化的组蛋白H4结合，而转录沉默的卵母细胞基因则不然。
J Biol Chem. 1998 Aug 14;273(33):20693-6. doi: 10.1074/jbc.273.33.20693.
5
Histone acetylation: influence on transcription, nucleosome mobility and positioning, and linker histone-dependent transcriptional repression.组蛋白乙酰化：对转录、核小体移动性和定位以及连接组蛋白依赖性转录抑制的影响。
EMBO J. 1997 Apr 15;16(8):2096-107. doi: 10.1093/emboj/16.8.2096.
6
Inhibition of 5S RNA transcription in vitro by nucleosome cores with low or high levels of histone acetylation.低水平或高水平组蛋白乙酰化的核小体核心在体外对5S RNA转录的抑制作用。
FEBS Lett. 1991 Aug 19;288(1-2):215-8. doi: 10.1016/0014-5793(91)81037-9.
7
The AT-rich flanks of the oocyte-type 5S RNA gene of Xenopus laevis act as a strong local signal for histone H1-mediated chromatin reorganization in vitro.非洲爪蟾卵母细胞型5S RNA基因富含AT的侧翼在体外作为组蛋白H1介导的染色质重组的强大局部信号。
Nucleic Acids Res. 1997 Feb 1;25(3):458-66. doi: 10.1093/nar/25.3.458.
8
Intra- and inter-nucleosomal interactions of the histone H4 tail revealed with a human nucleosome core particle with genetically-incorporated H4 tetra-acetylation.通过基因整合的H4四乙酰化人核小体核心颗粒揭示的组蛋白H4尾巴的核小体内和核小体间相互作用。
Sci Rep. 2015 Nov 26;5:17204. doi: 10.1038/srep17204.
9
Role of histone H1 as an architectural determinant of chromatin structure and as a specific repressor of transcription on Xenopus oocyte 5S rRNA genes.组蛋白H1作为染色质结构的构建决定因素以及非洲爪蟾卵母细胞5S rRNA基因转录的特异性阻遏物的作用。
Mol Cell Biol. 1998 Jul;18(7):3668-80. doi: 10.1128/MCB.18.7.3668.
10
Distribution of histone H4 modifications as revealed by a panel of specific monoclonal antibodies.一组特异性单克隆抗体所揭示的组蛋白H4修饰分布情况。
Chromosome Res. 2015 Dec;23(4):753-66. doi: 10.1007/s10577-015-9486-4. Epub 2015 Sep 5.

引用本文的文献

1
Research Progress on the Mechanism and Function of Histone Acetylation Regulating the Interaction between Pathogenic Fungi and Plant Hosts.组蛋白乙酰化调控病原真菌与植物宿主互作的机制与功能研究进展
J Fungi (Basel). 2024 Jul 26;10(8):522. doi: 10.3390/jof10080522.
2
In Vitro Models of Amyotrophic Lateral Sclerosis.体外肌萎缩侧索硬化症模型。
Cell Mol Neurobiol. 2023 Nov;43(8):3783-3799. doi: 10.1007/s10571-023-01423-8. Epub 2023 Oct 23.
3
Epigenetic Modulations for Prevention of Infectious Diseases in Shrimp Aquaculture.

本文引用的文献

1
Organization of Chromatin by Intrinsic and Regulated Phase Separation.染色质的固有和调控相分离组织。
Cell. 2019 Oct 3;179(2):470-484.e21. doi: 10.1016/j.cell.2019.08.037. Epub 2019 Sep 19.
2
Expanding and reprogramming the genetic code.扩展和重编程遗传密码。
Nature. 2017 Oct 4;550(7674):53-60. doi: 10.1038/nature24031.
3
Novel layers of RNA polymerase III control affecting tRNA gene transcription in eukaryotes.影响真核生物tRNA基因转录的RNA聚合酶III调控的新层次。
虾类水产养殖中传染病预防的表观遗传调控。
Genes (Basel). 2023 Aug 25;14(9):1682. doi: 10.3390/genes14091682.
4
Histone Acetyltransferase Rtt109 Regulates Development, Morphogenesis, and Citrinin Biosynthesis in .组蛋白乙酰转移酶Rtt109调控……中的发育、形态发生和桔霉素生物合成。 （原文此处不完整，缺少具体物种信息）
J Fungi (Basel). 2023 Apr 29;9(5):530. doi: 10.3390/jof9050530.
5
A transcriptional cycling model recapitulates chromatin-dependent features of noisy inducible transcription.转录循环模型再现了依赖染色质的噪声诱导转录的特征。
PLoS Comput Biol. 2022 Sep 9;18(9):e1010152. doi: 10.1371/journal.pcbi.1010152. eCollection 2022 Sep.
6
Histone acylations and chromatin dynamics: concepts, challenges, and links to metabolism.组蛋白酰化和染色质动力学：概念、挑战以及与代谢的联系。
EMBO Rep. 2021 Jul 5;22(7):e52774. doi: 10.15252/embr.202152774. Epub 2021 Jun 23.
Open Biol. 2017 Feb;7(2). doi: 10.1098/rsob.170001.
4
Nucleosome Positioning and NDR Structure at RNA Polymerase III Promoters.核小体定位和 RNA 聚合酶 III 启动子处的 NDR 结构。
Sci Rep. 2017 Feb 8;7:41947. doi: 10.1038/srep41947.
5
Quantitative Measurement of Histone Tail Acetylation Reveals Stage-Specific Regulation and Response to Environmental Changes during Drosophila Development.组蛋白尾部乙酰化的定量测量揭示了果蝇发育过程中的阶段特异性调控及对环境变化的反应。
Biochemistry. 2016 Mar 22;55(11):1663-72. doi: 10.1021/acs.biochem.5b01070. Epub 2016 Feb 18.
6
Intra- and inter-nucleosomal interactions of the histone H4 tail revealed with a human nucleosome core particle with genetically-incorporated H4 tetra-acetylation.通过基因整合的H4四乙酰化人核小体核心颗粒揭示的组蛋白H4尾巴的核小体内和核小体间相互作用。
Sci Rep. 2015 Nov 26;5:17204. doi: 10.1038/srep17204.
7
Comparative overview of RNA polymerase II and III transcription cycles, with focus on RNA polymerase III termination and reinitiation.RNA聚合酶II和III转录周期的比较概述，重点关注RNA聚合酶III的终止和重新起始。
Transcription. 2014;5(1):e27639. doi: 10.4161/trns.27369.
8
50 years of protein acetylation: from gene regulation to epigenetics, metabolism and beyond.50 年的蛋白质乙酰化研究：从基因调控到表观遗传学、代谢及其他领域。
Nat Rev Mol Cell Biol. 2015 Apr;16(4):258-64. doi: 10.1038/nrm3931. Epub 2014 Dec 30.
9
Chromatin accessibility: a window into the genome.染色质可及性：窥探基因组的窗口。
Epigenetics Chromatin. 2014 Nov 20;7(1):33. doi: 10.1186/1756-8935-7-33. eCollection 2014.
10
Expanded genetic code technologies for incorporating modified lysine at multiple sites.用于在多个位点掺入修饰赖氨酸的扩展遗传密码技术。
Chembiochem. 2014 Oct 13;15(15):2181-7. doi: 10.1002/cbic.201402266. Epub 2014 Sep 1.