College of Veterinary Medicine University of the Philippines Los Baños, College, Los Baños Laguna, 4031, Philippines.
UPLB Bee Program, University of the Philippines Los Baños, College, Los Baños Laguna, 4031, Philippines.
Exp Anim. 2021 May 13;70(2):185-193. doi: 10.1538/expanim.20-0118. Epub 2020 Nov 25.
Despite decades-long existence of the Philippine stingless bee industry, the biological activity of propolis from this native bee species (Tetragonula biroi Friese) remains poorly understood and sparingly investigated. Herein, we examined the potential anti-inflammatory efficacy of Philippine stingless bee propolis using the lambda (λ)-carrageenan-induced mice model of hind paw edema. Thirty (30), six-week-old, male ICR mice were randomly assigned into three treatment groups (n=10/group) as follows: distilled water group, diclofenac sodium group (10 mg/kg), and propolis group (100 mg/kg). All treatment were administered an hour prior to the injection of the phlogistic agent. As observed at 3 h post-injection, λ-carrageenan remarkably evoked the classical signs of hind paw edema exemplified grossly by swelling and hyperemia. The ameliorative effect of propolis became apparent at the onset of 6 h post-injection with a statistically significant finding evident at the 24-h period. This gross attenuation histologically correlated to a considerable and specific reduction of the dermal edema, which mirrored those of the diclofenac sodium group. Furthermore, both propolis and diclofenac sodium significantly attenuated the λ-carrageenan-induced increase in the protein expression levels of the pro-inflammatory cytokine tumor necrosis factor-α (TNF-α) depicting more than two-fold decrement relative to the distilled water group. Altogether, these suggest that Philippine stingless bee propolis also exhibited a promising in vivo anti-inflammatory property, which can be partly mediated through the inhibition of TNF-α.
尽管菲律宾无刺蜜蜂产业已经存在了几十年，但这种本土蜜蜂（Tetragonula biroi Friese）的蜂胶的生物活性仍知之甚少，研究也很少。在此，我们使用角叉菜胶诱导的小鼠后爪水肿模型来研究菲律宾无刺蜜蜂蜂胶的潜在抗炎功效。将 30 只 6 周龄雄性 ICR 小鼠随机分为三组（每组 10 只）：蒸馏水组、双氯芬酸钠组（10 mg/kg）和蜂胶组（100 mg/kg）。所有处理均在注射致炎剂前 1 小时进行。如在注射后 3 小时观察到的那样，角叉菜胶显著引起了后爪水肿的经典征象，表现为肿胀和充血。蜂胶的改善作用在注射后 6 小时开始显现，在 24 小时时出现了统计学上的显著差异。这种肉眼观察到的衰减在组织学上与真皮水肿的显著减少相关，这与双氯芬酸钠组的结果相似。此外，蜂胶和双氯芬酸钠均显著抑制了角叉菜胶诱导的促炎细胞因子肿瘤坏死因子-α（TNF-α）的蛋白表达水平增加，与蒸馏水组相比，TNF-α的表达水平降低了两倍以上。总之，这些结果表明菲律宾无刺蜜蜂蜂胶也表现出有希望的体内抗炎特性，这可能部分是通过抑制 TNF-α 来介导的。
