Institute of Microbiology, Heilongjiang Academy of Sciences, Harbin, China.
Institute of Food Research, Hezhou University, Hezhou, China.
Front Immunol. 2020 Nov 6;11:595700. doi: 10.3389/fimmu.2020.595700. eCollection 2020.
Recently, the immuno-enhancing potential of polysaccharide from (AAP) has been an area of research interest. However, the immune-stimulatory activity and mechanisms of AAP in immunosuppressive mice treated with cyclophosphamide (CTX) are still poorly understood. This study aimed to evaluate the immuno-enhancing effects of AAP and mine its possible mechanisms. Firstly, polysaccharides were isolated from and purified. Secondly, the immune-stimulatory activities of the first AAP fraction (AAP1) were evaluated in the CTX-treated mice. Results showed that AAP1 significantly enhanced immune organ indexes, remarkably stimulated IFN-γ, IL-2, IL-4, IL-10, and TNF-α levels in the serum, and dramatically up-regulated the mRNA levels of Claudin-1, Occludin and ZO-1. Compared to the CTX group, AAP1 administration restored the gut microbiota composition similar to that of the control group by decreasing the ratio of Firmicutes/Bacteroidetes and increasing the relative abundances of short-chain fatty acid-producing microbiota. This study provides useful information for its further application as an immune-stimulator in foods and drugs.
最近，（AAP）多糖的免疫增强潜力一直是研究热点。然而，用环磷酰胺（CTX）处理的免疫抑制小鼠中 AAP 的免疫刺激活性和机制仍知之甚少。本研究旨在评估 AAP 的免疫增强作用，并探讨其可能的机制。首先，从 中分离并纯化多糖。其次，评估了第一 AAP 级分（AAP1）在 CTX 处理的小鼠中的免疫刺激活性。结果表明，AAP1 显著增强免疫器官指数，显著刺激血清中 IFN-γ、IL-2、IL-4、IL-10 和 TNF-α 水平，并显著上调 Claudin-1、Occludin 和 ZO-1 的 mRNA 水平。与 CTX 组相比，AAP1 给药通过降低厚壁菌门/拟杆菌门的比例和增加产生短链脂肪酸的微生物的相对丰度，使肠道微生物群组成恢复到类似于对照组的水平。本研究为其在食品和药物中作为免疫刺激剂的进一步应用提供了有用的信息。
