Department of Chemical Engineering, Villanova University, Villanova, PA 19085, USA.
Int J Environ Res Public Health. 2022 May 1;19(9):5506. doi: 10.3390/ijerph19095506.
Antimicrobial resistance (AMR) is a serious public health issue. Due to resistance to current antibiotics and a low rate of development of new classes of antimicrobials, AMR is a leading cause of death worldwide. Listeria monocytogenes is a deadly foodborne pathogen that causes listeriosis for the immunocompromised, the elderly, and pregnant women. Unfortunately, antimicrobial resistance has been reported in L. monocytogenes. This study conducted the first comprehensive statistical analysis of L. monocytogenes isolate data from the National Pathogen Detection Isolate Browser (NPDIB) to identify the trends for AMR genes in L. monocytogenes. Principal component analysis was firstly used to project the multi-dimensional data into two dimensions. Hierarchical clustering was then used to identify the significant AMR genes found in L. monocytogenes samples and to assess changes during the period from 2010 through to 2021. Statistical analysis of the data identified fosX, lin, abc-f, and tet(M) as the four most common AMR genes found in L. monocytogenes. It was determined that there was no increase in AMR genes during the studied time period. It was also observed that the number of isolates decreased from 2016 to 2020. This study establishes a baseline for the ongoing monitoring of L. monocytogenes for AMR genes.
抗微生物药物耐药性（AMR）是一个严重的公共卫生问题。由于对现有抗生素的耐药性以及新类别的抗菌药物研发率低，AMR 是全球范围内导致死亡的主要原因。单核细胞增生李斯特菌是一种致命的食源性病原体，会导致免疫功能低下者、老年人和孕妇李斯特菌病。不幸的是，单核细胞增生李斯特菌已出现抗微生物药物耐药性。本研究首次对国家病原体检测分离物浏览器（NPDIB）中的单核细胞增生李斯特菌分离物数据进行了全面的统计分析，以确定单核细胞增生李斯特菌中 AMR 基因的趋势。首先使用主成分分析将多维数据投影到两个维度。然后使用层次聚类来识别单核细胞增生李斯特菌样本中发现的重要 AMR 基因，并评估 2010 年至 2021 年期间的变化。对数据的统计分析确定 fosX、lin、abc-f 和 tet(M) 为单核细胞增生李斯特菌中最常见的四个 AMR 基因。研究结果表明，在研究期间，AMR 基因没有增加。还观察到，从 2016 年到 2020 年，分离物的数量减少了。本研究为单核细胞增生李斯特菌抗微生物药物耐药性基因的持续监测奠定了基础。
