Brown H R, Goller N L, Thormar H, Rudelli R, Tourtellotte W W, Shapshak P, Boostanfar R, Wisniewski H M
New York State Office of Mental Retardation and Developmental Disabilities, Institute for Basic Research, Staten Island 10314.
Acta Neuropathol. 1987;75(2):123-30. doi: 10.1007/BF00687072.
Subacute sclerosing panencephalitis (SSPE) is a rare, fatal disease of children caused by a persistent measles virus infection of the central nervous system. A defect in synthesis of measles virus matrix (M) protein may be a factor in virus persistence in the brain. This study details attempts to detect expression of M protein in the brain of an SSPE patient, in the cell-associated virus isolated from this brain, and in brains of ferrets inoculated with the isolate. In situ hybridization with a tritiated cloned cDNA probe was used to search for RNA encoding M protein. Immunostaining with monospecific antiserum and the avidin-biotin-peroxidase technique was done to locate the polypeptide. The data obtained indicate that although nucleotide sequences coding for M protein were detected in the patient and ferret brains, expression of M protein in these tissues could not be detected. In the culture SSPE virus isolate, the results were the same until the infected cells were examined by electron microscopy and a very limited expression of M protein was revealed. This suggests either diminished synthesis and/or rapid degradation of M protein in this cell-associated virus strain.
亚急性硬化性全脑炎（SSPE）是一种由麻疹病毒持续感染中枢神经系统引起的儿童罕见致命疾病。麻疹病毒基质（M）蛋白合成缺陷可能是病毒在脑中持续存在的一个因素。本研究详细描述了检测SSPE患者脑内、从该脑部分离的细胞相关病毒以及接种该分离株的雪貂脑中M蛋白表达的尝试。使用氚标记的克隆cDNA探针进行原位杂交以寻找编码M蛋白的RNA。用单特异性抗血清和抗生物素蛋白-生物素-过氧化物酶技术进行免疫染色以定位该多肽。获得的数据表明，尽管在患者和雪貂脑中检测到了编码M蛋白的核苷酸序列，但在这些组织中未检测到M蛋白的表达。在培养的SSPE病毒分离株中，结果相同，直到通过电子显微镜检查感染细胞并发现M蛋白有非常有限的表达。这表明在该细胞相关病毒株中，M蛋白的合成减少和/或降解迅速。
