Laboratory of Reproductive Medicine and Molecular Endocrinology, Center for Translational Medicine (CEMT-BIOREN), Universidad de La Frontera, Temuco, Chile; Escuela de Medicina Veterinaria, Facultad de Recursos Naturales y Medicina Veterinaria, Universidad Santo Tomas, Chile.
Biotechnology of Animal and Human Reproduction (TechnoSperm), Unit of Cell Biology, Department of Biology, Institute of Food and Agricultural Technology, Faculty of Sciences, University of Girona, Girona, Spain.
Cryobiology. 2021 Feb;98:5-11. doi: 10.1016/j.cryobiol.2020.11.007. Epub 2020 Nov 25.
In swine, the use of frozen-thawed boar sperm for artificial insemination remains a suboptimal reproductive technology. Among the negative effects of cryopreservation on sperm cells, it is worth highlighting that cryopreservation causes irreversible alterations in motility and components of the sperm membrane as a result of dramatic changes in temperature (cooling/freezing curve) and osmolality. In addition, freeze-thawing may induce oxidative stress and increase the generation of reactive oxygen species (ROS) and nitrogen reactive species (RNS). While boar sperm cryopreservation has been reported to increase lipid peroxidation and the intracellular levels of hydrogen peroxide, less research on its impact on RNS has been conducted. Furthermore, previous studies have investigated the effects of supplementing cryopreservation media with antioxidants to counteract the deleterious effects of ROS and RNS. Antioxidants of synthetic origin or natural extracts have been used, with some showing noticeable and positive effects on functional sperm parameters both in vitro and in vivo. The aim of this review is to provide an update on the effect of different molecules with antioxidant capacity on the function of cryopreserved boar sperm.
在猪中，使用冷冻解冻的公猪精子进行人工授精仍然是一种不尽理想的生殖技术。在冷冻对精子细胞的负面影响中，值得强调的是，冷冻导致的温度（冷却/冷冻曲线）和渗透压的剧烈变化会使精子膜的运动和成分发生不可逆的改变。此外，冷冻-解冻可能会诱导氧化应激，增加活性氧（ROS）和氮活性物质（RNS）的产生。虽然已经有报道称猪精子冷冻保存会增加脂质过氧化和过氧化氢的细胞内水平，但对其对 RNS 的影响的研究较少。此外，以前的研究已经调查了在冷冻保存介质中添加抗氧化剂以抵消 ROS 和 RNS 的有害影响的效果。已经使用了合成来源或天然提取物的抗氧化剂，其中一些在体外和体内对功能精子参数都显示出明显的积极作用。本综述的目的是提供关于具有抗氧化能力的不同分子对冷冻保存公猪精子功能的影响的最新信息。
