Wang Jing, Zhang Ruiyuan, Ji Xiaoting, Wang Peipei, Ding Caifeng
Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science, Ministry of Education, Shandong Key Laboratory of Biochemical Analysis, Key Laboratory of Analytical Chemistry for Life Science in Universities of Shandong, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao, 266042, PR China.
Qingdao Central Hospital, Qingdao, 266042, China.
Anal Chim Acta. 2021 Jan 2;1141:206-213. doi: 10.1016/j.aca.2020.10.033. Epub 2020 Oct 19.
Herein, a dual-selective recognition and multi-enhanced surface-enhanced Raman scattering (SERS)-fluorescence dual mode detection platform is designed for the detection of circulating tumor cells (CTCs). The gold nanoflowers (AuNFs) substrate was synthesized and the CTCs were captured on the surface area of AuNFs/ITO substrate by aptamers modified. At the same time, the novel nanoprobe was designed, anti-EpCAM (AE) and trigger DNA were modified onto the surface of gold nanostars (AuNSs) through a PEG linker. The novel nanoprobe identified CTCs through the specific recognition reaction between AE and the cell epithelial adhesion molecule of the CTCs. The dual-recognition cellular mechanism of the aptamers and AE improves selectivity. Then, the complementary sequence (CS) hybridize with aptamers to release the captured CTCs into the culture medium. The number of CTCs released was detected by SERS and fluorescence. The limit of SERS detection was 5 cells/mL with a linear relationship from 5 to 200 cells/mL. The limit of fluorescence detection was 10 cells/mL with a linear relationship from 10 to 200 cells/mL. Thus, the developed CTCs detection platform demonstrates promising applications for clinical diagnosis.
在此，设计了一种双选择性识别和多重增强的表面增强拉曼散射（SERS）-荧光双模式检测平台，用于循环肿瘤细胞（CTC）的检测。合成了金纳米花（AuNFs）基底，并通过修饰的适体将CTCs捕获在AuNFs/ITO基底的表面区域。同时，设计了新型纳米探针，通过聚乙二醇接头将抗上皮细胞粘附分子（AE）和触发DNA修饰到金纳米星（AuNSs）表面。该新型纳米探针通过AE与CTCs的细胞上皮粘附分子之间的特异性识别反应来识别CTCs。适体和AE的双识别细胞机制提高了选择性。然后，互补序列（CS）与适体杂交，将捕获的CTCs释放到培养基中。通过SERS和荧光检测释放的CTCs数量。SERS检测限为5个细胞/mL，在5至200个细胞/mL范围内呈线性关系。荧光检测限为10个细胞/mL，在10至200个细胞/mL范围内呈线性关系。因此，所开发的CTCs检测平台在临床诊断中显示出有前景的应用。
