Medical Laboratory Techniques Department, Al-maarif University College, Iraq.
Department of Environment, College of Science, University of Al-Qadisiyah, Iraq.
Microb Pathog. 2021 Mar;152:104649. doi: 10.1016/j.micpath.2020.104649. Epub 2020 Nov 26.
Arcobacter spp colonize in human and animals intestine and cause food-associated infections. Hence, characterization of their virulence potential and health impacts is required. Our subject was isolation and characterization of Arcobacter spp, from meat marketplaces. A total of 1297 fresh raw cattle meat samples were purchased randomly from various marketplaces in Baghdad, Iraq. One-hundred and twenty isolates were identified, including Arcobacter butzleri (A. butzleri n = 100) and Arcobacter cryaerophilus (A. cryaerophilus n = 20). Susceptibility to antimicrobials was examined using Kirby-Bauer disc diffusion method. Molecular investigation of antibiotic resistance and virulence factors was also conducted using polymerase chain reaction (PCR) technique. Most of A. butzleri were resistant to tetracycline (72%), amoxicillin (69%), erythromycin (67%) and cefoxitin (66%), while 33% and 6% of them were resistant to ceftazidime and carbapenems, respectively. All were susceptible to gentamicin, colistin and fosfomycin. Fifty-five and nine isolates of A. butzleri and A. cryaerophilus were respectively multidrug-resistant (MDR). The existence of tetA, tetB, dfrA, sul1, bla and bla included 61%, 58%, 57%, 34%, 46% and 3%, respectively. The virulence genes cadF, irgA, tylA, cdtC and cdtA genes were detected in all the A. butzleri and A. cryaerophilus isolates. While, ciaB mviN and pldA genes were respectively detected in 91%, 88% and 84% of A. butzleri and 97%, 93% and 87% of A. cryaerophilus isolates. There was a significant relation between MDR and existence of virulence genes. Existence of pathogenic and drug-resistant- Arcobacter spp in raw meat is a threat for human health, necessitating confirmation of quality and safety of meat products.
空肠弯曲菌属定居在人和动物的肠道中，引起与食物相关的感染。因此，需要对其毒力潜力和健康影响进行特征描述。我们的研究对象是从肉市场中分离和鉴定空肠弯曲菌属。从伊拉克巴格达的各个市场随机购买了 1297 份新鲜生牛肉样本。共鉴定出 120 株分离株，包括空肠弯曲菌（A. butzleri n = 100）和嗜冷弯曲菌（A. cryaerophilus n = 20）。采用 Kirby-Bauer 纸片扩散法检测抗菌药物敏感性。还使用聚合酶链反应（PCR）技术进行了抗生素耐药性和毒力因子的分子研究。大多数空肠弯曲菌对四环素（72%）、阿莫西林（69%）、红霉素（67%）和头孢西丁（66%）耐药，而 33%和 6%的菌株分别对头孢他啶和碳青霉烯类耐药。所有菌株均对庆大霉素、黏菌素和磷霉素敏感。55 株和 9 株空肠弯曲菌和嗜冷弯曲菌分别为多重耐药（MDR）。61%、58%、57%、34%、46%和 3%的分离株分别携带 tetA、tetB、dfrA、sul1、bla 和 bla 基因。所有空肠弯曲菌和嗜冷弯曲菌分离株均检测到 cadF、irgA、tylA、cdtC 和 cdtA 毒力基因。而 ciaB mviN 和 pldA 基因分别在 91%、88%和 84%的空肠弯曲菌和 97%、93%和 87%的嗜冷弯曲菌中检测到。MDR 与毒力基因的存在之间存在显著关系。生肉中存在致病性和耐药性-空肠弯曲菌属对人类健康构成威胁，需要确认肉类产品的质量和安全性。
