Finch G R, Stiles M E, Smith D W
Department of Civil Engineering, University of Alberta, Edmonton, Canada.
Appl Environ Microbiol. 1987 Dec;53(12):2894-6. doi: 10.1128/aem.53.12.2894-2896.1987.
Selective and nonselective growth media were evaluated at two incubation temperatures, 35 and 44.5 degrees C, for the recovery of a nalidixic acid-resistant marker strain of Escherichia coli ATCC 11775 by membrane filtration from ozonated 0.05 M phosphate buffer (pH 6.9). There were significantly fewer bacteria recovered with the standard m-FC agar when compared with the same growth medium prepared without bile salts and rosolic acid. This effect was particularly noticeable at the elevated incubation temperature of 44.5 degrees C. These findings are contrary to previous work which concluded that the standard American Public Health Association membrane filtration procedure is suitable for recovery of fecal coliform indicator bacteria from ozonated wastewater.
在35和44.5摄氏度这两个培养温度下,对选择性和非选择性生长培养基进行了评估,以通过膜过滤从经臭氧处理的0.05M磷酸盐缓冲液(pH 6.9)中回收耐萘啶酸的大肠杆菌ATCC 11775标记菌株。与不含胆盐和玫瑰红酸的相同生长培养基相比,使用标准m-FC琼脂回收的细菌明显较少。在44.5摄氏度的较高培养温度下,这种效果尤为明显。这些发现与之前的研究结果相反,之前的研究得出结论,美国公共卫生协会的标准膜过滤程序适用于从经臭氧处理的废水中回收粪大肠菌群指示菌。