Jeong Geum-Jae, Kim Do-Kyun, Cho Kyung-Jin, Choi Eun-Jin, Jung Won-Kyo, Khan Fazlurrahman, Kim Young-Mog
Department of Food Science and Technology, Pukyong National University, Busan 48513, Republic of Korea.
Marine Integrated Biomedical Technology Center, The National Key Research Institutes in Universities, Pukyong National University, Busan 48513, Republic of Korea.
J Microbiol Biotechnol. 2025 Jul 18;35:e2505029. doi: 10.4014/jmb.2505.05029.
Surfactants play a critical role in diverse industrial applications. However, concerns over the environmental persistence and toxicity of synthetic variants have prompted a shift toward sustainable biosurfactants. In this study, the surface properties of a biosurfactant produced by HH2 isolated from , a traditional Korean fermented seafood, were investigated to evaluate its industrial applicability. The biosurfactant exhibited consistent and stable emulsifying performance across a wide range of salinity (3-18%), temperature (30-80°C), and pH (4-10) conditions. Emulsification assays with various hydrocarbon substrates revealed a performance equal to or better than that of the synthetic surfactant Triton X-100. While the biosurfactant generated a lower initial foam volume than Triton X-100, it maintained a significantly higher foam stability over 60 min, highlighting its suitability for applications that benefit from persistent and low-volume foaming. Optical microscopy showed that the biosurfactant produced smaller and more uniform bubbles than those of Triton X-100. Furthermore, the producing strain demonstrated strong hydrophobic interactions with hydrocarbons, such as hexadecane, toluene, and pyrene, supporting its potential utility in hydrocarbon-rich environments. Collectively, these findings highlight the multifunctional surface and functional activity and environmental robustness of the HH2-derived biosurfactant, indicating its potential as a sustainable and effective alternative for diverse industrial applications, including food processing, cosmetics, and environmental remediation.
表面活性剂在多种工业应用中发挥着关键作用。然而，对合成变体的环境持久性和毒性的担忧促使人们转向可持续的生物表面活性剂。在本研究中，对从韩国传统发酵海鲜中分离出的HH2所产生的生物表面活性剂的表面性质进行了研究，以评估其工业适用性。该生物表面活性剂在广泛的盐度（3-18%）、温度（30-80°C）和pH值（4-10）条件下表现出一致且稳定的乳化性能。对各种烃类底物的乳化试验表明，其性能与合成表面活性剂Triton X-100相当或更好。虽然该生物表面活性剂产生的初始泡沫体积比Triton X-100低，但在60分钟内保持了显著更高的泡沫稳定性，突出了其在受益于持久和低体积泡沫的应用中的适用性。光学显微镜显示，该生物表面活性剂产生的气泡比Triton X-100产生的更小且更均匀。此外，生产菌株与十六烷、甲苯和芘等烃类表现出强烈的疏水相互作用，支持其在富含烃类环境中的潜在用途。总的来说，这些发现突出了HH2衍生的生物表面活性剂的多功能表面和功能活性以及环境稳健性，表明其作为食品加工、化妆品和环境修复等多种工业应用的可持续和有效替代品的潜力。
