Lei Li, Yan Ran, Chen Shougang, Hao Xiangping, Dou Wenwen, Liu Hu, Guo Zhanhu, Kilula Dan, Seok Ilwoo
School of Materials Science and Engineering, Ocean University of China, Qingdao 266100, China.
School of Materials Science and Engineering, Ocean University of China, Qingdao 266100, China.
Mater Sci Eng C Mater Biol Appl. 2021 Jan;118:111414. doi: 10.1016/j.msec.2020.111414. Epub 2020 Aug 24.
An intelligent narrow pH-triggered multilayer film was prepared on magnesium alloys, aiming to solve the implant infections during the implantation period and improve the corrosion resistance of magnesium alloys. The encapsulation of ibuprofen by chitosan (IBU@CS) makes the release of IBU sensitive to narrow pH (pH 6.8-7.4). Positive charged IBU@CS was assembled with heparin (Hep) to fabricate (Hep/IBU@CS) film on AZ31 alloys using layer-by-layer method. The microstructure, composition and anticorrosion properties of the film were characterized by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR) and electrochemical experiments. Cellular activity was studied by MTT cell viability assay. The results showed that the Hep/IBU@CS multilayer films improved the corrosion resistance of magnesium alloys. The in vitro test demonstrated that the release of IBU in the film presented narrow pH sensitivity. The films showed no obvious signs of cytotoxicity conformed by the MTT assay and presented antibacterial properties. These preliminary results demonstrate the potential use of the Hep/IBU@CS multilayer films on magnesium-based implants.
在镁合金上制备了一种智能窄pH触发多层膜，旨在解决植入期间的植入物感染问题，并提高镁合金的耐腐蚀性。壳聚糖对布洛芬的包封（IBU@CS）使IBU的释放对窄pH值（pH 6.8 - 7.4）敏感。将带正电荷的IBU@CS与肝素（Hep）组装，采用层层法在AZ31合金上制备（Hep/IBU@CS）膜。通过扫描电子显微镜（SEM）、傅里叶变换红外光谱（FT-IR）和电化学实验对膜的微观结构、组成和防腐性能进行了表征。通过MTT细胞活力测定法研究细胞活性。结果表明，Hep/IBU@CS多层膜提高了镁合金的耐腐蚀性。体外试验表明，膜中IBU的释放呈现窄pH敏感性。MTT试验证实这些膜没有明显的细胞毒性迹象，并具有抗菌性能。这些初步结果证明了Hep/IBU@CS多层膜在镁基金属植入物上的潜在应用。
