Zhang Fan, Bambharoliya Tushar, Xie Yu, Liu Laijun, Celik Hakan, Wang Lu, Akkus Ozan, King Martin W
Wilson College of Textiles, North Carolina State University, Raleigh, NC, USA.
Wilson College of Textiles, North Carolina State University, Raleigh, NC, USA.
Mater Sci Eng C Mater Biol Appl. 2021 Jan;118:111418. doi: 10.1016/j.msec.2020.111418. Epub 2020 Aug 22.
Tissue-engineered small caliber vascular grafts have attracted much research attention as a viable alternative to traditional vascular grafts with their biocompatibility and potential to achieve complete healing. However, the major challenge is to fabricate a scaffold with both satisfactory mechanical properties and fast endothelialization. In this study, a hybrid tubular vascular tissue engineered scaffold has been circular-knitted using novel electrochemically aligned collagen (ELAC) filaments plied together with traditional poly(lactic acid) (PLA) yarn. The collagen component was able to promote the recruitment and proliferation of endothelial cells by increasing the initial cell adhesion 10-fold and the eventual cell population 3.2 times higher than the PLA scaffold alone. At the same time, the PLA yarn was able to provide sufficient mechanical strength and structural stability, as well as facilitate scaffold fabrication on high speed textile production equipment. The tubular hybrid scaffold exhibited excellent bursting strength (1.89 ± 0.43 MPa) and suture retention strength (10.86 ± 0.49 N), and had comparable compliance (3.98 ± 1.94%/100 mmHg) to that of the coronary artery (3.8 ± 0.3%/100 mmHg) under normotensive pressure. With its excellent mechanical and biological performance, this prototype hybrid scaffold is a promising candidate for the construction of a clinically successful and easily translatable tissue-engineered small caliber vascular graft.
组织工程小口径血管移植物作为传统血管移植物的可行替代品，因其生物相容性和实现完全愈合的潜力而备受研究关注。然而，主要挑战在于制造一种兼具令人满意的机械性能和快速内皮化能力的支架。在本研究中，一种混合管状血管组织工程支架已通过将新型电化学排列的胶原蛋白（ELAC）细丝与传统聚乳酸（PLA）纱线捻合在一起进行圆编而成。胶原蛋白成分能够通过将初始细胞粘附力提高10倍以及使最终细胞数量比单独的PLA支架高出3.2倍，来促进内皮细胞的募集和增殖。同时，PLA纱线能够提供足够的机械强度和结构稳定性，并便于在高速纺织生产设备上制造支架。管状混合支架表现出优异的爆破强度（1.89±0.43兆帕）和缝线保留强度（10.86±0.49牛），并且在正常血压下与冠状动脉的顺应性（3.8±0.3%/100毫米汞柱）相当（3.98±1.94%/100毫米汞柱）。凭借其优异的机械和生物学性能，这种原型混合支架是构建临床上成功且易于转化的组织工程小口径血管移植物的有希望的候选者。
