Zhou Yuanyuan, Li Xinhui, Yan He
School of Food Science and Engineering, South China University of Technology, Guangzhou 510000, China.
Department of Microbiology, University of Wisconsin-La Crosse, 1725 State Street, La Crosse, WI 54601, USA.
Antibiotics (Basel). 2020 Nov 24;9(12):839. doi: 10.3390/antibiotics9120839.
China is one of the largest producers of pigs and pork in the world. However, large-scale studies on pig-associated in relation to healthy pigs, diseased pigs and environment are scarce. The objective of the present study was to characterize and compare isolates from healthy pigs, diseased pigs and environment through antimicrobial susceptibility testing, multiple locus sequence typing, typing, and antimicrobial resistance gene screening. Results showed all isolates were susceptible to linezolid and vancomycin. However, 66.7% (104/156) isolates were multidrug-resistant by displaying resistance to three or more antibiotics and high rates of resistance to penicillin, tetracycline, clindamycin, and clarithromycin were observed. Of the 20 multilocus sequence types (STs) identified among the isolates, ST9, ST188, and ST7 were most commonly isolated from healthy pigs and environment, while ST1 was most commonly isolated from diseased pigs. In total, 17 types were represented among the isolates, while t4792 was most commonly isolated from diseased pigs and t899, t189 were most commonly isolated from healthy pigs and environment. In conclusion, the genotypic and epidemiology characteristics observed among the isolates suggest pigs and pork could be important players in dissemination.
中国是世界上最大的生猪和猪肉生产国之一。然而，关于与健康猪、患病猪及环境相关的猪源[具体对象未明确]的大规模研究却很匮乏。本研究的目的是通过抗菌药物敏感性测试、多位点序列分型、[未明确的分型方式]分型以及抗菌药物耐药基因筛查，对从健康猪、患病猪和环境中分离出的[具体对象未明确]进行特征描述和比较。结果显示，所有分离株对利奈唑胺和万古霉素敏感。然而，66.7%（104/156）的分离株对三种或更多种抗生素耐药，表现为多重耐药，且观察到对青霉素、四环素、克林霉素和克拉霉素的耐药率很高。在分离出的菌株中鉴定出的20种多位点序列类型（STs）中，ST9、ST188和ST7最常从健康猪和环境中分离得到，而ST1最常从患病猪中分离得到。在分离株中总共出现了17种[未明确的类型]，其中t4792最常从患病猪中分离得到，t899、t189最常从健康猪和环境中分离得到。总之，在分离株中观察到的基因型和流行病学特征表明，猪和猪肉可能是[未明确的传播对象]传播的重要载体。
