Al-Tamimi Maram, Al-Massarani Shaza M, El-Gamal Ali A, Basudan Omer A, Abdel-Kader Maged S, Abdel-Mageed Wael M
Department of Pharmacognosy, College of Pharmacy, King Saud University, PO. Box 2457, Riyadh 11451, Saudi Arabia.
Department of Pharmacognosy, Faculty of Pharmacy, Mansoura University, El-Mansoura 35516, Egypt.
Plants (Basel). 2020 Nov 24;9(12):1632. doi: 10.3390/plants9121632.
A new dihydroisocoumarin glucoside, vacillanoside (), and two new anthrone -glycosides microdantin derivatives; vacillantin A () and B (), together with nine known compounds belonging to the anthraquinone, anthrone and isocoumarin groups were isolated from the leaves of . The structures were determined based on spectroscopic evidence including 1D and 2D nuclear magnetic resonance (NMR) spectroscopy and high resolution mass spectrometry (HRESIMS) data, along with comparisons to reported data. The leaves were used to extract compounds with different solvents. The extracts were tested for antioxidant activity with a variety of in vitro tests including 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonate (ABTS), ferric reducing antioxidant power assay (FRAP), superoxide, and nitric oxide radical scavenging assays. The dichloromethane fraction was most active, displaying significant free radical scavenging activity. The -butanol fraction also showed notable activity in all assays. Therefore, these findings support the potential use of leaves as an antioxidant medication due to the presence of polyphenolic compounds.
从某植物的叶子中分离出一种新的二氢异香豆素葡萄糖苷，即摇摆苷（），以及两种新的蒽酮 - 糖苷微丹宁衍生物；摇摆亭A（）和B（），还有九种属于蒽醌、蒽酮和异香豆素类的已知化合物。基于包括一维和二维核磁共振（NMR）光谱以及高分辨率质谱（HRESIMS）数据在内的光谱证据，并与已报道的数据进行比较，确定了这些化合物的结构。用不同溶剂从叶子中提取化合物。通过多种体外试验，包括2,2 - 二苯基 - 1 - 苦基肼（DPPH）、2,2'- 偶氮 - 双（3 - 乙基苯并噻唑啉 - 6 - 磺酸盐）（ABTS）、铁还原抗氧化能力测定（FRAP）、超氧化物和一氧化氮自由基清除试验，对提取物的抗氧化活性进行了测试。二氯甲烷部分活性最高，表现出显著的自由基清除活性。正丁醇部分在所有试验中也显示出显著活性。因此，由于存在多酚类化合物，这些发现支持了将该植物叶子用作抗氧化药物的潜在用途。
