Robertson M D, Seaton A, Raeburn J A, Milne L J
Institute of Occupational Medicine, Edinburgh, U.K.
J Med Vet Mycol. 1987 Dec;25(6):389-96. doi: 10.1080/02681218780000471.
Previous studies have shown that spores of Aspergillus fumigatus inhibit phagocytosis and killing by macrophages and polymorphonuclear leucocytes. In order to identify the mechanisms of this interference with host defences, we have examined the effects of A. fumigatus spore diffusates on phagocytic cell function. For comparison, we have used spore diffusates of the non-pathogenic fungus Penicillium ochrochloron. The diffusates of A. fumigatus reduced the number of human polymorphonuclear leukocytes migrating towards a known chemoattractant by approximately 50% (p less than 0.001). In addition spore diffusates of A. fumigatus significantly decreased (p less than 0.001) the capacity of primed mouse peritoneal exudate cells to spread on glass. Spore diffusates of P. ochrochloron showed no comparable inhibitory effects. These studies have shown that spore diffusates of A. fumigatus inhibit the movement of the phagocytic cell membrane and are thus able to interfere with a primary function of phagocytic cells.
以往研究表明，烟曲霉孢子可抑制巨噬细胞和多形核白细胞的吞噬作用及杀伤功能。为了确定这种对宿主防御干扰的机制，我们研究了烟曲霉孢子扩散物对吞噬细胞功能的影响。作为对照，我们使用了非致病性真菌黄绿青霉的孢子扩散物。烟曲霉的扩散物使趋化因子诱导下的人多形核白细胞迁移数量减少了约50%（p<0.001）。此外，烟曲霉的孢子扩散物显著降低了致敏小鼠腹腔渗出细胞在玻璃上的铺展能力（p<0.001）。黄绿青霉的孢子扩散物未显示出类似的抑制作用。这些研究表明，烟曲霉的孢子扩散物可抑制吞噬细胞膜的运动，从而干扰吞噬细胞的主要功能。
