低温生物学在制霉菌素微生物测定中的应用。
The application of cryobiology to the microbiological assay of nystatin.
作者信息
Cosgrove R F, Beezer A E, Miles R J
出版信息
J Pharm Pharmacol. 1979 Feb;31(2):83-6. doi: 10.1111/j.2042-7158.1979.tb13437.x.
Improvements in the reproducibility of nystatin agar diffusion assays have been achieved by the use of liquid nitrogen stored inocula and deep frozen standard stock solutions. The overall percentage variability of the assay has been reduced from over 5% with daily prepared standards and inocula to around 1% with a frozen inocula and to 0.6% with a combination of frozen inocula and standards. The implications of these improvements in the standardization of nystatin assays, and microbiological assays generally are discussed.
通过使用液氮保存的接种物和深度冷冻的标准储备溶液，制霉菌素琼脂扩散试验的可重复性得到了提高。该试验的总体变异百分比已从每日制备标准品和接种物时的超过5%降至使用冷冻接种物时的约1%，以及使用冷冻接种物和标准品组合时的0.6%。本文讨论了这些改进对制霉菌素试验标准化以及一般微生物学试验的意义。
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