苍耳属植物的酚类化合物及其对 CCl4 诱导的大鼠肝毒性的肝保护潜力的水提物和醇提物。
Phenolic compounds and hepatoprotective potential of Anastatica hierochuntica ethanolic and aqueous extracts against CCl4-induced hepatotoxicity in rats.
机构信息
Department of Food Science and Human Nutrition, College of Agriculture and Veterinary Medicine, Qassim University, Buraydah 51452, Saudi Arabia.
Food Technology Department, Faculty of Agriculture, Benha University, 13736 Moshtohor, Qaliuobia, Egypt.
出版信息
J Tradit Chin Med. 2020 Dec;40(6):947-955. doi: 10.19852/j.cnki.jtcm.2020.06.006.
OBJECTIVE
To investigate the effect of Anastatica hierochuntica ethanolic (KEE), aqueous (KAE) extracts, and their combination against CCl4-induced hepatotoxicity in rats.
METHODS
The HPLC analysis for KEE and KAE was quantitatively carried out. Biochemical liver markers, antioxidant enzymes, and histopathological alterations were examined then total hepatoprotection potential was calculated.
RESULTS
Among 9 identified phenolic compounds (PC) in KEA, sinapic acid was the highest while syringic acid was the highest among 21 identified PC in KAE. Six flavonoids were identified in KEE and two in KAE using HPLC, respectively. Oral administration of KEE, KAE, and KEE + KAE at 250 mg/kg body weight significantly reduced aspartate aminotransferase (AST), alanine aminotransferase (ALT) levels, alkaline phosphatase (ALP), total bilirubin (TBILI), and also attenuated histopathological changes. Additionally, they reduced malondialdehyde (MOD), restored reduced-glutathione (GSH), and enhanced superoxide dismutase (SOD) levels. KEE, KAE, and KEE+KAE protected the liver from CCl4-hepatotoxicity as they mainly attenuating oxidative stress. Total hepatoprotection was about 128.3%, 114.5%, and 103.8% for KEE, KAE, and KEE+KAE, respectively.
CONCLUSION
Biochemical observations, supplemented by histopathological examination revealed that AH affords extract-depending protection against CCl4-hepatotoxicity.
目的
研究 Anastatica hierochuntica 乙醇（KEE）、水（KAE）提取物及其组合对 CCl4 诱导的大鼠肝毒性的影响。
方法
对 KEE 和 KAE 进行 HPLC 分析。检测生化肝标志物、抗氧化酶和组织病理学改变，然后计算总肝保护潜力。
结果
在 KEA 中鉴定的 9 种酚类化合物（PC）中，咖啡酸含量最高，而 KAE 中鉴定的 21 种 PC 中丁香酸含量最高。使用 HPLC 分别在 KEE 和 KAE 中鉴定出 6 种黄酮类化合物和 2 种黄酮类化合物。以 250mg/kg 体重口服 KEE、KAE 和 KEE+KAE 可显著降低天冬氨酸氨基转移酶（AST）、丙氨酸氨基转移酶（ALT）水平、碱性磷酸酶（ALP）、总胆红素（TBILI），并减轻组织病理学变化。此外，它们还降低了丙二醛（MOD），恢复了还原型谷胱甘肽（GSH），并增强了超氧化物歧化酶（SOD）水平。KEE、KAE 和 KEE+KAE 可保护肝脏免受 CCl4 肝毒性，主要是通过减轻氧化应激。KEE、KAE 和 KEE+KAE 的总肝保护率分别约为 128.3%、114.5%和 103.8%。
结论
生化观察结果，辅以组织病理学检查表明，AH 提供了依赖提取物的 CCl4 肝毒性保护作用。
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