Laboratoire Kastler Brossel, Sorbonne Université, École Normale Supérieure-Paris Sciences et Lettres (PSL) Research University, Centre National de la Recherche Scientifique (CNRS) UMR 8552, Collège de France, 24 rue Lhomond, 75005, Paris, France.
Laboratoire de Physique de l'École Normale Supérieure, Université Paris Sciences et Lettres (PSL), Centre National de la Recherche Scientifique (CNRS), Sorbonne Université, Université Paris-Diderot, Sorbonne Paris Cité, 24 rue Lhomond, 75005, Paris, France.
Nat Commun. 2020 Dec 1;11(1):6154. doi: 10.1038/s41467-020-19696-8.
In biological microscopy, light scattering represents the main limitation to image at depth. Recently, a set of wavefront shaping techniques has been developed in order to manipulate coherent light in strongly disordered materials. The Transmission Matrix approach has shown its capability to inverse the effect of scattering and efficiently focus light. In practice, the matrix is usually measured using an invasive detector or low-resolution acoustic guide stars. Here, we introduce a non-invasive and all-optical strategy based on linear fluorescence to reconstruct the transmission matrices, to and from a fluorescent object placed inside a scattering medium. It consists in demixing the incoherent patterns emitted by the object using low-rank factorizations and phase retrieval algorithms. We experimentally demonstrate the efficiency of this method through robust and selective focusing. Additionally, from the same measurements, it is possible to exploit memory effect correlations to image and reconstruct extended objects. This approach opens up a new route towards imaging in scattering media with linear or non-linear contrast mechanisms.
在生物显微镜中，光散射是对深度进行成像的主要限制。最近，已经开发出了一系列波前整形技术，以便在强无序材料中操控相干光。传输矩阵方法已经证明了其反转散射效应并有效聚焦光的能力。在实践中，矩阵通常使用侵入式探测器或低分辨率声导星进行测量。在这里，我们引入了一种基于线性荧光的非侵入式全光学策略，用于重构传输矩阵，从置于散射介质中的荧光物体到该物体，并从该物体进行重构。它包括使用低秩因子分解和相位恢复算法来分解物体发出的非相干模式。我们通过稳健和选择性聚焦实验证明了该方法的效率。此外，从相同的测量中，可以利用记忆效应相关性来对扩展物体进行成像和重建。这种方法为具有线性或非线性对比度机制的散射介质中的成像开辟了新途径。
