Alshahrani Abdullah, Bin-Shuwaish Mohammed S, Al-Hamdan Rana S, Almohareb Thamer, Maawadh Ahmed M, Al Deeb Modhi, Alhenaki Aasem M, Abduljabbar Tariq, Vohra Fahim
Department of Prosthetic Dental Sciences, College of Dentistry, King Saud University, Riyadh, Saudi Arabia.
Department of Restorative Dental Sciences, College of Dentistry, King Saud University, Riyadh, Saudi Arabia.
J Appl Biomater Funct Mater. 2020 Jan-Dec;18:2280800020966936. doi: 10.1177/2280800020966936.
The study aimed to assess graphene oxide (GO) adhesive and its dentin interaction using scanning electron microscopy (SEM), MicroRaman spectroscopy and Microtensile bond strength (μTBS).
Experimental GOA and control adhesives (CA) were fabricated. Presence of GO within the experimental adhesive resin was assessed using SEM and Micro-Raman spectroscopy. Ninety specimens were prepared, sixty teeth were utilized for μTBS, twenty for SEM analysis of interface for CA and GOA and ten were assessed using microRaman spectroscopy. Each specimen was sectioned and exposed dentine was conditioned (35% phosphoric acid) for 10 s. The surface was coated twice with adhesive (15 s) and photopolymerized (20 s). Composite build-up on specimen was photo-polymerized. Among the bonded specimens, thirty specimen were assessed using Micro-Raman spectrometer, SEM and energy dispersive X-ray spectroscopy (EDX), whereas remaining specimens were divided in to three sub-groups ( = 10) based on the storage of 24 h, 8 weeks and 16 weeks. μTBS testing was performed at a crosshead speed of 0.5 mm/min using a microtensile tester. The means of μ-tbs were analyzed using ANOVA and post hoc Tukey multiple comparisons test.
No significant difference in μTBS of CA and GOA was observed. Storage time presented a significant interaction on the μTBS ( < 0.01). The highest and lowest μTBS was evident in CA (30.47 (3.55)) at 24 h and CA (22.88 (3.61)) at 18 weeks. Micro-Raman analysis identified peaks of 1200 cm-1 to 1800 cm1, D and G bands of GO nanoparticles in the resin. Uniform distribution of graphene oxide nanoparticles was present at the adhesive and hybrid layer.
GO showed interaction within adhesive and tooth dentin similar to CA, along with formation of hybrid layer. In ideal conditions (absence of nanoleakage), graphene oxide modified adhesive shows comparable bond strength and durability of resin dentine bond.
本研究旨在通过扫描电子显微镜（SEM）、显微拉曼光谱和微拉伸粘结强度（μTBS）评估氧化石墨烯（GO）粘结剂及其与牙本质的相互作用。
制备实验性GOA粘结剂和对照粘结剂（CA）。使用SEM和显微拉曼光谱评估实验性粘结树脂中GO的存在情况。制备了90个样本，60颗牙齿用于μTBS测试，20颗用于CA和GOA界面的SEM分析，10颗用显微拉曼光谱进行评估。将每个样本切片，对暴露的牙本质用（35%磷酸）处理10秒。表面用粘结剂涂覆两次（15秒）并光固化（20秒）。样本上的复合树脂堆积进行光固化。在粘结样本中，30个样本用显微拉曼光谱仪、SEM和能量色散X射线光谱（EDX）进行评估，而其余样本根据储存24小时、8周和16周分为三个亚组（每组 = 10）。使用微拉伸测试仪以0.5毫米/分钟的十字头速度进行μTBS测试。使用方差分析和事后Tukey多重比较检验分析μTBS的平均值。
未观察到CA和GOA的μTBS有显著差异。储存时间对μTBS有显著影响（P < 0.01）。CA在24小时时μTBS最高（30.47（3.55）），在18周时最低（22.88（3.61））。显微拉曼分析确定了树脂中GO纳米颗粒在1200 cm-1至1800 cm1处的峰、D带和G带。粘结剂层和混合层中存在氧化石墨烯纳米颗粒的均匀分布。
GO在粘结剂和牙本质之间显示出与CA类似的相互作用，并形成了混合层。在理想条件下（无纳米渗漏），氧化石墨烯改性粘结剂显示出与树脂 - 牙本质粘结相当的粘结强度和耐久性。
