Department of Pharmacy, Faculty of Medical Sciences, University of Kragujevac, Svetozara Markovica 69, Kragujevac, 34 000, Serbia.
Department of Physiology, Faculty of Medical Sciences, University of Kragujevac, Svetozara Markovica 69, Kragujevac, 34 000, Serbia.
Mol Cell Biochem. 2019 Oct;460(1-2):151-164. doi: 10.1007/s11010-019-03577-w. Epub 2019 Jul 6.
Diallyl trisulfide (DATS) is distinguished as the most potent polysulfide isolated from garlic. The aim of our study was to investigate effects of oral administration of DATS on healthy and diabetic rats, with special attention on heart function. Rats were randomly divided into four groups: CTRL (healthy rats), DATS (healthy rats treated with DATS), DM (diabetic rats), DM + DATS (diabetic rats treated with DATS). DATS (40 mg/kg of body weight) was administered every other day for 3 weeks, at the end of which rats underwent echocardiography, glycemic measurement and redox status assessment. Isolated rat hearts were subjected to 30 min global ischemia and 60 min reperfusion, after which heart tissue was counterstain with hematoxylin and eosin and cardiac Troponin T staining (cTnT), while expression of Bax, B cell lymphoma 2 (Bcl-2), caspase-3, caspase-9 and superoxide dismutase-2 were examined in the left ventricle. DATS treatment significantly reduced blood glucose levels of diabetic rats, and improved cardiac function recovery, diminished oxidation status, attenuated cardiac remodeling and inhibited myocardial apoptosis in healthy and diabetic rats. DATS treatment causes promising cardioprotective effects on ex vivo-induced ischemia/reperfusion (I/R) injury in diabetic and healthy rat heart probably mediated by inhibited myocardial apoptosis. Moreover, appropriate DATS consumption may provide potential co-therapy or prevention of hyperglycemia and various cardiac complications in rats with DM.
二烯丙基三硫醚（DATS）是从大蒜中分离出的最有效的多硫化物。我们的研究目的是研究 DATS 对健康和糖尿病大鼠的影响，特别关注心脏功能。大鼠随机分为四组：CTRL（健康大鼠）、DATS（用 DATS 治疗的健康大鼠）、DM（糖尿病大鼠）、DM+DATS（用 DATS 治疗的糖尿病大鼠）。DATS（40mg/kg 体重）每隔一天给药一次，共 3 周，在最后一次给药后，大鼠进行超声心动图、血糖测量和氧化还原状态评估。分离的大鼠心脏进行 30 分钟的整体缺血和 60 分钟的再灌注，之后用苏木精和伊红以及心肌肌钙蛋白 T 染色（cTnT）对心脏组织进行复染，同时检查左心室中 Bax、B 细胞淋巴瘤 2（Bcl-2）、caspase-3、caspase-9 和超氧化物歧化酶-2 的表达。DATS 治疗显著降低了糖尿病大鼠的血糖水平，并改善了心脏功能恢复，减轻了氧化状态，减弱了健康和糖尿病大鼠的心脏重构，抑制了心肌细胞凋亡。DATS 治疗对糖尿病和健康大鼠心脏的体外诱导缺血/再灌注（I/R）损伤具有有希望的心脏保护作用，可能是通过抑制心肌细胞凋亡介导的。此外，适当的 DATS 消耗可能为糖尿病大鼠提供潜在的协同治疗或预防高血糖和各种心脏并发症的方法。
