Corbic Milena, Jakovljevic Vladimir, Nikolic Marina, Jeremic Nevena, Bradic Jovana, Novakovic Jovana, Kocovic Aleksandar, Savic Maja, Tadic Vanja, Zugic Ana, Krivokapic Milos, Daskalovic Branislava, Mihajlovic Katarina, Sretenovic Jasmina
Clinic of Psychiatry, AMEOS Klinikum Hildesheim, Goslarsche Landstr.60, 31135, Hildesheim, Germany.
Department of Physiology, Faculty of Medical Sciences, University of Kragujevac, Svetozara Markovica 69, 34000, Kragujevac, Serbia.
Mol Cell Biochem. 2025 Jun;480(6):3783-3798. doi: 10.1007/s11010-025-05215-0. Epub 2025 Feb 2.
Psoriasis has been considered a systemic immune-mediated disease that can affect function of the heart. However, certain herbal therapy approaches may nullify side effects of psoriasis on the heart. The aim of this study was to investigate the cardioprotective effects of Galium verum extract administration of the heart of psoriatic rats. The study included 24 Wistar albino male rats, divided into 4 groups: control (CTRL), G.verum (GV), psoriasis (PSORI), and psoriasis with G.verum (PSORI + GV). Seven-day topical application of 5% imiquimod cream was used for induction of psoriasis. After induction, animals were received 125 mg/kg G.verum extract for 4 weeks. Isolated hearts were perfused on the Langendorff apparatus and measured: dp/dt max/min, SLVP, DLVP, HR and coronary flow. The oxidative stress biomarkers: TBARS, NO2, O2- and H2O2 were measured in coronary venous effluent. Isolated hearts were fixed and stained with H/E and Picro-sirius red staining. Psoriasis decreased cardiac contractility and relaxation and increased SLVP and DLVP at all perfusion pressure. Treatment with G.verum in psoriasis rats improved contractility and relaxation of the heart and rise SLVP and DLVP. In PSORI + GV group, the decrease of oxidative stress biomarkers were observed in comparison to PSORI group. Diameter and cross-section area of cardiomyocytes were increased in PSORI and PSORI + GV groups compared to the control. Collagen content was increased in PSORI group by 283% and in PSORI + GV group by 188% compared to control. Treatment with G.verum extract exhibited a positive effect on cardiac function, morphometry and redox state of heart of psoriatic rats.
银屑病被认为是一种可影响心脏功能的系统性免疫介导疾病。然而，某些草药治疗方法可能会消除银屑病对心脏的副作用。本研究的目的是调查给予猪殃殃提取物对银屑病大鼠心脏的心脏保护作用。该研究包括24只雄性Wistar白化大鼠，分为4组：对照组（CTRL）、猪殃殃组（GV）、银屑病组（PSORI）和银屑病加猪殃殃组（PSORI + GV）。采用5%咪喹莫特乳膏局部应用7天来诱导银屑病。诱导后，动物接受125 mg/kg猪殃殃提取物，持续4周。将离体心脏在Langendorff装置上进行灌注，并测量：dp/dt max/min、左心室收缩末期压（SLVP）、左心室舒张末期压（DLVP）、心率（HR）和冠状动脉血流量。在冠状静脉流出液中测量氧化应激生物标志物：丙二醛（TBARS）、二氧化氮（NO2）、超氧阴离子（O2-）和过氧化氢（H2O2）。将离体心脏固定并用苏木精/伊红（H/E）和苦味酸天狼星红染色。银屑病降低了心脏收缩力和舒张功能，并在所有灌注压力下增加了SLVP和DLVP。用猪殃殃治疗银屑病大鼠可改善心脏的收缩力和舒张功能，并提高SLVP和DLVP。与PSORI组相比，PSORI + GV组氧化应激生物标志物减少。与对照组相比，PSORI组和PSORI + GV组心肌细胞直径和横截面积增加。与对照组相比，PSORI组胶原蛋白含量增加283%，PSORI + GV组增加188%。给予猪殃殃提取物对银屑病大鼠心脏的心脏功能、形态学和氧化还原状态具有积极作用。
