Beijing Key Laboratory for Forest Pest Control, Beijing Forestry University, Beijing 100083, China.
Sino-French Joint Laboratory for Invasive Forest Pests in Eurasia, Beijing Forestry University-French National Research Institute for Agriculture, Food and Environment (INRAE), Beijing 100083, China.
Int J Mol Sci. 2020 Nov 22;21(22):8845. doi: 10.3390/ijms21228845.
An obligate mutualistic relationship exists between the fungus and woodwasp . The fungus digests lignin in the host pine, providing essential nutrients for the growing woodwasp larvae. However, the functional properties of this symbiosis are poorly described. In this study, we identified, cloned, and characterized 14 laccase genes from . These genes encoded proteins of 508 to 529 amino acids and contained three typical copper-oxidase domains, necessary to confer laccase activity. Besides, we performed molecular docking and dynamics simulation of the laccase proteins in complex with lignin compounds (monomers, dimers, trimers, and tetramers). AaLac2, AaLac3, AaLac6, AaLac8, and AaLac10 were found that had low binding energies with all lignin model compounds tested and three of them could maintain stability when binding to these compounds. Among these complexes, amino acid residues ALA, GLN, LEU, PHE, PRO, and SER were commonly present. Our study reveals the molecular basis of laccases interacting with lignin, which is essential for understanding how the fungus provides nutrients to These findings might also provide guidance for the control of by informing the design of enzyme mutants that could reduce the efficiency of lignin degradation.
在真菌和蛀木蜂之间存在着一种强制性的互利共生关系。真菌在宿主松树上消化木质素，为生长中的蛀木蜂幼虫提供必要的营养。然而，这种共生关系的功能特性描述得很差。在这项研究中，我们从 中鉴定、克隆和表征了 14 个漆酶基因。这些基因编码 508 到 529 个氨基酸的蛋白质，含有三个典型的铜氧化酶结构域，这是赋予漆酶活性所必需的。此外，我们对漆酶蛋白与木质素化合物（单体、二聚体、三聚体和四聚体）复合物进行了分子对接和动力学模拟。发现 AaLac2、AaLac3、AaLac6、AaLac8 和 AaLac10 与所有测试的木质素模型化合物具有较低的结合能，其中三种在与这些化合物结合时能够保持稳定性。在这些复合物中，常见的氨基酸残基有 ALA、GLN、LEU、PHE、PRO 和 SER。我们的研究揭示了漆酶与木质素相互作用的分子基础，这对于理解真菌如何为 提供营养至关重要。这些发现也可能为通过设计能够降低木质素降解效率的酶突变体来控制 提供指导。
